Journal Article

Metagenome Analysis Identified Novel Microbial Diversity of Sandy Soils Surrounded by Natural Lakes and Artificial Water Points in King Salman Bin Abdulaziz Royal Natural Reserve, Saudi Arabia

Al-Awthan, Yahya S., Alharbi, Basmah M., Shohdi, Wael M., Almutairi, Fahad M., Alatawi, Abdulaziz S., Alatawi, Fuad A., Khafaga, Tamer, Fakhry, Amal M., Mir, Rashid

2024

Overview

Background: Soil microbes play a vital role in the ecosystem as they are able to carry out a number of vital tasks. Additionally, metagenomic studies offer valuable insights into the composition and functional potential of soil microbial communities. Furthermore, analyzing the obtained data can improve agricultural restoration practices and aid in developing more effective environmental management strategies. Methodology: In November 2023, sandy soil samples were collected from ten sites of different geographical areas surrounding natural lakes and artificial water points in the Tubaiq conservation area of King Salman Bin Abdulaziz Royal Natural Reserve (KSRNR), Saudi Arabia. In addition, genomic DNA was extracted from the collected soil samples, and 16S rRNA sequencing was conducted using high-throughput Illumina technology. Several computational analysis tools were used for gene prediction and taxonomic classification of the microbial groups. Results: In this study, sandy soil samples from the surroundings of natural and artificial water resources of two distinct natures were used. Based on 16S rRNA sequencing, a total of 24,563 OTUs were detected. The metagenomic information was then categorized into 446 orders, 1036 families, 4102 genera, 213 classes, and 181 phyla. Moreover, the phylum Pseudomonadota was the most dominant microbial community across all samples, representing an average relative abundance of 34%. In addition, Actinomycetes was the most abundant class (26%). The analysis of clustered proteins assigned to COG categories provides a detailed understanding of the functional capabilities and adaptation of microbial communities in soil samples. Amino acid metabolism and transport were the most abundant categories in the soil environment. Conclusions: Metagenome analysis of sandy soils surrounding natural lakes and artificial water points in the Tubaiq conservation area of KSRNR (Saudi Arabia) has unveils rich microbial activity, highlighting the complex interactions and ecological roles of microbial communities in these environments.

• Publisher: MDPI AG
• Subject: 16S rRNA gene / Actinomycetes / Agricultural ecosystems / Amino acids / Aquatic resources / bacterial community / Biodiversity / Biological activity / Conservation areas / Conserved sequence / DNA sequencing / Ecosystems / Environmental management / Environmental protection / Environmental restoration / Gene sequencing / Genera / Genomes / Lakes / metagenome analysis / Metagenomics / Microbial activity / Microbiomes / Microorganisms / natural reserve / Nature reserves / next-generation sequencing / Protection and preservation / Protein transport / Proteins / Relative abundance / RNA / rRNA 16S / Sandy soils / Saudi Arabia / Software / Soil analysis / Soil classification / Soil conservation / Soil ecology / Soil environment / Soil improvement / Soil microorganisms / Strategic planning (Business) / Taxonomy / Technology assessment / Water conservation / Water resources