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Construction of a single-cell transcriptome atlas for Pogostemon cablin embryoids reveals PcNAC048 as a dual regulator coordinating lateral root morphogenesis and patchouli alcohol biosynthesis
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Construction of a single-cell transcriptome atlas for Pogostemon cablin embryoids reveals PcNAC048 as a dual regulator coordinating lateral root morphogenesis and patchouli alcohol biosynthesis
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Construction of a single-cell transcriptome atlas for Pogostemon cablin embryoids reveals PcNAC048 as a dual regulator coordinating lateral root morphogenesis and patchouli alcohol biosynthesis
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Journal Article
Construction of a single-cell transcriptome atlas for Pogostemon cablin embryoids reveals PcNAC048 as a dual regulator coordinating lateral root morphogenesis and patchouli alcohol biosynthesis
2025
Overview
Pogostemon cablin
Benth. (
P. cablin
) is an annual aromatic medicinal plant. In this study, anthers were cultured in vitro during the microspore development stage of
P. cablin
, inducing somatic embryogenesis. Globular somatic embryos (GSE), heart-shaped somatic embryos, torpedo-shaped somatic embryos, and cotyledonary somatic embryos (CSE) were observed and isolated. Single-cell RNA sequencing was then employed to generate single-cell maps for GSE and CSE. Using reported marker genes, a total of eight cell types were identified. Pseudo-temporal analysis reconstructed the continuous differentiation trajectory of apical meristem cells and epidermal cells. Further investigation identified PcNAC048 as a putative transcription factor that regulates embryonic cell differentiation. Gene expression analysis showed
PcNAC048
is expressed in different tissues of
P. cablin
and responds to abiotic stress. Transgenic studies in
Arabidopsis thaliana
showed that
PcNAC048
can promote lateral root development. Results from yeast one
-
hybrid and dual luciferase assays showed that PcNAC048 can interact with the promoter of the patchouli alcohol synthase gene (
PcPTS
) and inhibit its activity. Transient overexpression and virus-induced gene silencing (VIGS) analysis further confirmed that
PcNAC048
can negatively regulate the biosynthesis of patchouli alcohol. Overall, this study provides theoretical support for germplasm development and regulation of medicinal compounds in
P. cablin
.
In patchouli (
Pogostemon cablin
), anther-derived somatic embryos were profiled by single-cell RNA-seq to define eight cell types, identifying PcNAC048 that drives embryonic cell differentiation, promotes lateral root development in Arabidopsis, and negatively regulates patchouli alcohol biosynthesis.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/22
/ 38/77
/ Alcohol
/ Anthers
/ Biomedical and Life Sciences
/ Cells
/ Embryos
/ Gene Expression Regulation, Plant
/ Genomics
/ Leaves
/ Plant Roots - growth & development
/ Plants, Genetically Modified
/ Pogostemon - growth & development
/ Transcription Factors - genetics
