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Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil

by Petrie, James R. , Silva, Claudio , Belide, Srinivas , Shrestha, Pushkar , Buzza, Greg , Gao, Wenxiang , Green, Allan G. , Zhou, Xue-Rong , Mansour, Maged P. , Mulder, Roger J. , Lester, Geraldine , Devine, Malcolm D. , Cogan, Noel O. I. , Nichols, Peter D. , de Feyter, Robert , Tian, Lijun , Singh, Surinder P. , Kennedy, Yoko , McAllister, Jason , Liu, Qing , Gororo, Nelson , Leonforte, Antonio

in Algae / Biosynthesis / Brassica / Brassica napus / Canola / Cloning / Crops / Deregulation / DHA / Docosahexaenoic acid / Eicosapentaenoic acid / elite event / Embryos / Fatty acids / field trial / Fish oils / Genetic modification / Genetic transformation / Genetically engineered microorganisms / Lectins / Linolenic acid / Oilseeds / Oleic acid / Plant Science / Polyunsaturated fatty acids / Rape plants / Regulatory approval / Seeds / ω3 long-chain polyunsaturated fatty acid

2020

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Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil

2020

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Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil

2020

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Journal Article

Development of a Brassica napus (Canola) Crop Containing Fish Oil-Like Levels of DHA in the Seed Oil

Petrie, James R.,

Silva, Claudio,

Belide, Srinivas,

Shrestha, Pushkar,

Buzza, Greg,

Gao, Wenxiang,

Green, Allan G.,

Zhou, Xue-Rong,

Mansour, Maged P.,

Mulder, Roger J.,

Lester, Geraldine,

Devine, Malcolm D.,

Cogan, Noel O. I.,

Nichols, Peter D.,

de Feyter, Robert,

Tian, Lijun,

Singh, Surinder P.,

Kennedy, Yoko,

McAllister, Jason,

Liu, Qing,

Gororo, Nelson,

Leonforte, Antonio

2020

Overview

Plant seeds have long been promoted as a production platform for novel fatty acids such as the ω3 long-chain (≥ C20) polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) commonly found in fish oil. In this article we describe the creation of a canola ( Brassica napus ) variety producing fish oil-like levels of DHA in the seed. This was achieved by the introduction of a microalgal/yeast transgenic pathway of seven consecutive enzymatic steps which converted the native substrate oleic acid to α-linolenic acid and, subsequently, to EPA, docosapentaenoic acid (DPA) and DHA. This paper describes construct design and evaluation, plant transformation, event selection, field testing in a wide range of environments, and oil profile stability of the transgenic seed. The stable, high-performing event NS-B50027-4 produced fish oil-like levels of DHA (9–11%) in open field trials of T3 to T7 generation plants in several locations in Australia and Canada. This study also describes the highest seed DHA levels reported thus far and is one of the first examples of a deregulated genetically modified crop with clear health benefits to the consumer.

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Publisher

Frontiers Media SA,Frontiers Media S.A

Subject

/ Canola

/ Cloning

/ Crops