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Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq
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Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq
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Journal Article
Introducing synthetic thermostable RNase inhibitors to single-cell RNA-seq
2024
Overview
Single-cell RNA-sequencing (scRNAseq) is revolutionizing biomedicine, propelled by advances in methodology, ease of use, and cost reduction of library preparation. Over the past decade, there have been remarkable technical improvements in most aspects of single-cell transcriptomics. Yet, little to no progress has been made in advancing RNase inhibition despite maintained RNA integrity being critical during cell collection, storage, and cDNA library generation. Here, we demonstrate that a synthetic thermostable RNase inhibitor (SEQURNA) yields single-cell libraries of equal or superior quality compared to ubiquitously used protein-based recombinant RNase inhibitors (RRIs). Importantly, the synthetic RNase inhibitor provides additional unique improvements in reproducibility and throughput, enables new experimental workflows including retained RNase inhibition throughout heat cycles, and can reduce the need for dry-ice transports. In summary, replacing RRIs represents a substantial advancement in the field of single-cell transcriptomics.
Effective RNase control is crucial in single-cell transcriptomics. Here, the authors introduce a synthetic, thermostable RNase inhibitor that enhances RNA stability and provides greater workflow flexibility in single-cell RNA-seq library preparation.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
