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Spatiotemporal dynamics of the nuclear pore complex transport barrier resolved by high-speed atomic force microscopy
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Spatiotemporal dynamics of the nuclear pore complex transport barrier resolved by high-speed atomic force microscopy
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Journal Article
Spatiotemporal dynamics of the nuclear pore complex transport barrier resolved by high-speed atomic force microscopy
2016
Overview
Nuclear pore complexes (NPCs) are biological nanomachines that mediate the bidirectional traffic of macromolecules between the cytoplasm and nucleus in eukaryotic cells. This process involves numerous intrinsically disordered, barrier-forming proteins known as phenylalanine-glycine nucleoporins (FG Nups) that are tethered inside each pore. The selective barrier mechanism has so far remained unresolved because the FG Nups have eluded direct structural analysis within NPCs. Here, high-speed atomic force microscopy is used to visualize the nanoscopic spatiotemporal dynamics of FG Nups inside
Xenopus
laevis
oocyte NPCs at timescales of ∼100 ms. Our results show that the cytoplasmic orifice is circumscribed by highly flexible, dynamically fluctuating FG Nups that rapidly elongate and retract, consistent with the diffusive motion of tethered polypeptide chains. On this basis, intermingling FG Nups exhibit transient entanglements in the central channel, but do not cohere into a tightly crosslinked meshwork. Therefore, the basic functional form of the NPC barrier is comprised of highly dynamic FG Nups that manifest as a central plug or transporter when averaged in space and time.
High-speed atomic force microscopy can visualize the dynamics of phenylalanine-glycine nucleoporins inside nuclear pore complexes and reveals the selective barrier mechanism within these molecular machines.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
