Journal Article

Molecular characterization and expression of type-I interferon gene in Labeo rohita

Mukherjee, S. C, Parhi, Janmejay, Saxena, Gopalkrishna, Sahoo, Lopamudra, Makesh, M

2014

Overview

Genes coding for type-I interferon (I-IFN) has been cloned from Labeo rohita, a commercially important and widely cultured fish in India and South East Asia. In the present study, full-length gene of I-IFN was amplified and sequenced. The sequence analysis revealed that I-IFN consists of 1,786 bp genomic sequence with four introns and five exons and an ORF of 546 bp encoding for a putative protein of 181 amino acids. The mature protein has a molecular weight of 18.97 kDa and consists of 158 amino acids and a signal peptide of 23 amino acids at the N terminus. The sequence carries I-IFN signature motif, one glycosylation site, two conserved cystine amino acids and other conserved amino acids. The sequence showed highest similarity to that of Cyprinus carpio (84 %). In silico analysis of the rohu I-IFN protein was done using various bioinformatic tools. The constitutive expression of I-IFN gene was found to be more in spleen compared to gill and kidney in real time PCR assay. Expression of I-IFN increased about 20-fold in cultured kidney cell 2 h after induction with poly I:C and showed maximum expression at 8 h post-induction.

• Publisher: Springer-Verlag,Springer Netherlands,Springer Nature B.V
• Subject: Amino Acid Sequence / amino acids / Animal Anatomy / Animal Biochemistry / Animals / Base Sequence / bioinformatics / Biomedical and Life Sciences / computer simulation / Cyprinus carpio / cystine / exons / farmed fish / Fish / Fishes - genetics / Freshwater / Gene Expression / genes / glycosylation / Histology / India / Interferon / Interferon Type I - genetics / interferons / introns / kidneys / Labeo rohita / Life Sciences / Models, Molecular / Molecular biology / Molecular Sequence Data / molecular weight / Morphology / open reading frames / Phylogeny / polyinosinic-polycytidylic acid / Polymerase Chain Reaction / Protein Conformation / quantitative polymerase chain reaction / Sequence Alignment / sequence analysis / Sequence Analysis, DNA / signal peptide / South East Asia / spleen