Journal Article

Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae

Foubert, Imogen, Muylaert, Koenraad, Ryckebosch, Eline

2012

Overview

An optimized procedure for extraction of total and non-polar lipids from microalgae is proposed. The effects of solvent, pretreatment (lyophilization, inactivation of lipases, and addition of antioxidants) and cell-disruption (liquid nitrogen, sonication, and bead beating) on total lipid content, lipid class, and fatty acid composition were examined. Chloroform–methanol 1:1 was shown to be the best solvent mixture for extraction of total lipids from microalgae. When performing this extraction, lyophilized algae can be used, no pretreatment with isopropanol to inactivate the lipases is needed and addition of antioxidants is not necessary. Furthermore, cell-disruption is not essential, although in that case two extractions must be performed in series to ensure that, irrespective of the microalgal species, all lipids are extracted. Determination of non-polar lipid content should be performed by separation of the total lipid extract on an SPE column. Extraction using petroleum ether is only appropriate when a bead beater is used for pretreatment.

• Publisher: Springer-Verlag,Springer,Springer Nature B.V
• Subject: Agriculture / Algae / Antioxidants / Biological and medical sciences / Biomaterials / Biotechnology / Cell‐disruption / Chemistry / Chemistry and Materials Science / Chloroform / Extraction processes / Fat content / Fat industries / Fatty acid composition / Fatty acids / Food engineering / Food industries / Food Science / Freeze-drying / Fundamental and applied biological sciences. Psychology / General aspects / Inactivation / Industrial Chemistry/Chemical Engineering / Lipid extraction / Lipids / Microalgae / Nitrogen / Oil / Optimization / Original Paper / petroleum ether / Pretreatment / Solvent mixture / Solvents / Sonication / Triacylglycerol lipase