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Journal Article
dsRNAEngineer: a web-based tool of comprehensive dsRNA design for pest control
2025
Overview
We provide a one-stop online platform, namely, dsRNAEngineer, to help users optimize dsRNA for RNAi-based pest control.dsRNAEngineer incorporates a range of pest and non-pest species to enable large-scale transcriptome-level analysis for dsRNA design.dsRNAEngineer contains four functionalities, namely, screen-target, on-target, off-target, and multiple-target functions, to design rational dsRNAs that sufficiently target pests but are safe for non-target organisms.
Over the past two decades, many double-stranded (ds)-RNAs have been synthesized to silence target genes for exploration of gene functions in pests. Some of these dsRNAs are lethal to pests, leading to a new category of pesticides. The generation of these environmentally friendly pesticides requires precise in silico design of dsRNA molecules that target pests but not non-pest organisms. Current efforts in dsRNA design focus mainly on the analysis of the target gene sequence, lacking comprehensive analysis of all transcripts of the whole transcriptome per given species, causing low efficiency and imprecise dsRNA target exploration. To address these limitations, we created the dsRNAEngineer online platform (https://dsrna-engineer.cn), which allows comprehensive and rational dsRNA design, incorporating hundreds of pest and non-pest transcriptomes. Developed functionalities include screen-target (screen conserved genes for cotargets of various pest species), on-target, off-target, and multi-target to generate optimal dsRNA for precise pest control.
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dsRNAEngineer incorporates hundreds of pest and non-pest transcriptomes to provide comprehensive dsRNA design for pest control. With the application of several RNA-based pesticides in the field, dsRNAEngineer illustrates a framework for designing pest-specific and biosafe dsRNA, which will greatly promote the development of RNAi biotechnology as a pest control strategy.
Over the past two decades, RNAi biotechnology has been intensively investigated for pest control, and several products have been registered and applied in the field. However, current methods of double-stranded (ds)RNA design lack a comprehensive analysis of all transcripts at the transcriptome level for both pest and non-pest species. To solve these problems, our timely developed tool, dsRNAEngineer (https://dsrna-engineer.cn), which has four functionalities (screen-target, on-target, off-target, and multi-target functions) was developed to help users design rational dsRNAs that sufficiently target pests but are otherwise safe for non-target organisms, based on large-scale transcriptome-level analysis of both pest and non-pest species. With the emergence of RNA pesticides, we believe that dsRNAEngineer can be considered as a gold standard in dsRNA design to promote pest control based on RNAi biotechnology.
Publisher
Elsevier Ltd,Elsevier Limited
Subject
