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Heparanase activates the syndecan-syntenin-ALIX exosome pathway
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Heparanase activates the syndecan-syntenin-ALIX exosome pathway
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Journal Article
Heparanase activates the syndecan-syntenin-ALIX exosome pathway
2015
Overview
Exosomes are secreted vesicles of endosomal origin involved in signaling processes. We recently showed that the syndecan heparan sulfate proteoglycans control the biogenesis of exosomes through their interaction with synten- in-1 and the endosomai-sorting complex required for transport accessory component ALIX. Here we investigated the role of heparanase, the only mammalian enzyme able to cleave heparan sulfate internally, in the syndecan-synten- in-ALIX exosome biogenesis pathway. We show that heparanase stimulates the exosomal secretion of syntenin-1, syn- decan and certain other exosomal cargo, such as CD63, in a concentration-dependent manner. In contrast, exosomal CD9, CD81 and flotillin-1 are not affected. Conversely, reduction of endogenous heparanase reduces the secretion of syntenin-l-containing exosomes. The ability of heparanase to stimulate exosome production depends on syntenin-1 and ALIX. Syndecans, but not glypicans, support exosome biogenesis in heparanase-exposed cells. Finally, hepara- nase stimulates intraluminal budding of syndecan and syntenin-1 in endosomes, depending on the syntenin-ALIX in- teraction. Taken together, our findings identify heparanase as a modulator of the syndecan-syntenin-ALIX pathway, fostering endosomal membrane budding and the biogenesis of exosomes by trimming the heparan sulfate chains on syndecans. In addition, our data suggest that this mechanism controls the selection of specific cargo to exosomes.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Biomedical and Life Sciences
/ Calcium-Binding Proteins - biosynthesis
/ Calcium-Binding Proteins - genetics
/ Cell Cycle Proteins - biosynthesis
/ Cell Cycle Proteins - genetics
/ Endosomal Sorting Complexes Required for Transport - biosynthesis
/ Endosomal Sorting Complexes Required for Transport - genetics
/ Heparitin Sulfate - metabolism
/ Humans
/ Membrane Proteins - biosynthesis
/ Original
/ Sulfates
/ 乙酰肝素酶
/ 哺乳动物
/ 复杂控制
/ 浓度依赖性
/ 生物合成途径
/ 硫酸乙酰肝素
/ 聚糖
/ 酶激活
