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Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

by Weierstall, Uwe , James, Daniel , Fromme, Petra , Seuring, Carolin , Schwander, Peter , Milathianaki, Despina , Gati, Cornelius , Zatsepin, Nadia , Heymann, Michael , Wang, Dingjie , Schmidt, Marius , Henning, Robert , Spence, John C. H. , Moffat, Keith , Basu, Shibom , Koglin, Jason E. , Hunter, Mark , Conrad, Chelsie , Chapman, Henry N. , Yefanov, Oleksandr , Srajer, Vukica , Ourmazd, Abbas , Frank, Matthias , Williams, Garth J. , Kupitz, Christopher , Tenboer, Jason , Barty, Anton , Oberthuer, Dominik , Coe, Jesse , Scales, Jennifer , Pande, Kanupriya , Boutet, Sébastien , Fromme, Raimund , Roy-Chowdhury, Shatabdi , Van Thor, Jasper J. , Grant, Thomas

in Bacterial Proteins - chemistry / Bacterial Proteins - ultrastructure / BASIC BIOLOGICAL SCIENCES / Chromophores / Crystallography / Crystallography, X-Ray - methods / Crystals / Diffraction patterns / Electron density / Laser beams / Lasers / Molecules / OTHER INSTRUMENTATION / Photocatalysis / Photons / Photoreceptors, Microbial - chemistry / Photoreceptors, Microbial - ultrastructure / Protein Conformation / Proteins / Synchrotrons / Time Factors

2014

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Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

2014

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Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

2014

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Journal Article

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein

Weierstall, Uwe,

James, Daniel,

Fromme, Petra,

Seuring, Carolin,

Schwander, Peter,

Milathianaki, Despina,

Gati, Cornelius,

Zatsepin, Nadia,

Heymann, Michael,

Wang, Dingjie,

Schmidt, Marius,

Henning, Robert,

Spence, John C. H.,

Moffat, Keith,

Basu, Shibom,

Koglin, Jason E.,

Hunter, Mark,

Conrad, Chelsie,

Chapman, Henry N.,

Yefanov, Oleksandr,

Srajer, Vukica,

Ourmazd, Abbas,

Frank, Matthias,

Williams, Garth J.,

Kupitz, Christopher,

Tenboer, Jason,

Barty, Anton,

Oberthuer, Dominik,

Coe, Jesse,

Scales, Jennifer,

Pande, Kanupriya,

Boutet, Sébastien,

Fromme, Raimund,

Roy-Chowdhury, Shatabdi,

Van Thor, Jasper J.,

Grant, Thomas

2014

Overview

Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecuies. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

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Publisher

American Association for the Advancement of Science,The American Association for the Advancement of Science,AAAS

Subject

Bacterial Proteins - chemistry

/ Bacterial Proteins - ultrastructure

/ BASIC BIOLOGICAL SCIENCES

/ Chromophores

/ Crystallography

/ Crystallography, X-Ray - methods

/ Crystals