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Whole-central nervous system functional imaging in larval Drosophila
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Whole-central nervous system functional imaging in larval Drosophila
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Whole-central nervous system functional imaging in larval Drosophila
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Journal Article
Whole-central nervous system functional imaging in larval Drosophila
2015
Overview
Understanding how the brain works in tight concert with the rest of the central nervous system (CNS) hinges upon knowledge of coordinated activity patterns across the whole CNS. We present a method for measuring activity in an entire, non-transparent CNS with high spatiotemporal resolution. We combine a light-sheet microscope capable of simultaneous multi-view imaging at volumetric speeds 25-fold faster than the state-of-the-art, a whole-CNS imaging assay for the isolated
Drosophila
larval CNS and a computational framework for analysing multi-view, whole-CNS calcium imaging data. We image both brain and ventral nerve cord, covering the entire CNS at 2 or 5 Hz with two- or one-photon excitation, respectively. By mapping network activity during fictive behaviours and quantitatively comparing high-resolution whole-CNS activity maps across individuals, we predict functional connections between CNS regions and reveal neurons in the brain that identify type and temporal state of motor programs executed in the ventral nerve cord.
To understand how neuronal networks function, it is important to measure neuronal network activity at the systems level. Here Lemon
et al
. develop a framework that combines a high-speed multi-view light-sheet microscope, a whole-CNS imaging assay and computational tools to demonstrate simultaneous functional imaging across the entire isolated
Drosophila
larval CNS.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/63
/ 14/69
/ 631/378
/ 64/24
/ Animals
/ Brain
/ Central Nervous System - anatomy & histology
/ Central Nervous System - physiology
/ Drosophila melanogaster - anatomy & histology
/ Humanities and Social Sciences
/ Image Processing, Computer-Assisted - methods
/ Imaging
/ Insects
/ Mapping
/ Science
/ Software
