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Virtual screening as a tool to discover new β-haematin inhibitors with activity against malaria parasites
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Virtual screening as a tool to discover new β-haematin inhibitors with activity against malaria parasites
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Journal Article
Virtual screening as a tool to discover new β-haematin inhibitors with activity against malaria parasites
2020
Overview
Malaria remains a major public health problem. With the loss of antimalarials to resistance, the malaria burden will likely continue for decades. New antimalarial scaffolds are crucial to avoid cross-resistance. Here, we present the first structure based virtual screening using the β-haematin crystal as a target for new inhibitor scaffolds by applying a docking method. The ZINC15 database was searched for compounds with high binding affinity with the surface of the β-haematin crystal using the PyRx Virtual Screening Tool. Top-ranked compounds predicted to interact with β-haematin were submitted to a second screen applying
in silico
toxicity and drug-likeness predictions using Osiris DataWarrior. Fifteen compounds were purchased for experimental testing. An NP-40 mediated β-haematin inhibition assay and parasite growth inhibition activity assay were performed. The benzoxazole moiety was found to be a promising scaffold for further development, showing intraparasitic haemozoin inhibition using a cellular haem fractionation assay causing a decrease in haemozoin in a dose dependent manner with a corresponding increase in exchangeable haem. A β-haematin inhibition hit rate of 73% was found, a large enrichment over random screening, demonstrating that virtual screening can be a useful and cost-effective approach in the search for new haemozoin inhibiting antimalarials.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Animals
/ Antimalarials - pharmacology
/ Cell Survival - drug effects
/ Hemeproteins - antagonists & inhibitors
/ Humanities and Social Sciences
/ Malaria
/ Molecular Docking Simulation
/ Plasmodium falciparum - drug effects
/ Protozoan Proteins - antagonists & inhibitors
/ Protozoan Proteins - metabolism
/ Science
/ Toxicity
