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DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
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DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
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Journal Article
DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
2022
Overview
The promise of single-objective light-sheet microscopy is to combine the convenience of standard single-objective microscopes with the speed, coverage, resolution and gentleness of light-sheet microscopes. We present DaXi, a single-objective light-sheet microscope design based on oblique plane illumination that achieves: (1) a wider field of view and high-resolution imaging via a custom remote focusing objective; (2) fast volumetric imaging over larger volumes without compromising image quality or necessitating tiled acquisition; (3) fuller image coverage for large samples via multi-view imaging and (4) higher throughput multi-well imaging via remote coverslip placement. Our instrument achieves a resolution of 450 nm laterally and 2 μm axially over an imaging volume of 3,000 × 800 × 300 μm. We demonstrate the speed, field of view, resolution and versatility of our instrument by imaging various systems, including
Drosophila
egg chamber development, zebrafish whole-brain activity and zebrafish embryonic development – up to nine embryos at a time.
The DaXi single-objective light-sheet microscope achieves fast, high-quality imaging of large volumes. DaXi’s design allows increased scanning range without sacrificing imaging speed or quality, multiview imaging and versatile sample mounting.
Publisher
Nature Publishing Group US,Nature Publishing Group
