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Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
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Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
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Journal Article
Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2
2020
Overview
The COVID-19 pandemic has highlighted that new diagnostic technologies are essential for controlling disease transmission. Here, we develop SHINE (Streamlined Highlighting of Infections to Navigate Epidemics), a sensitive and specific diagnostic tool that can detect SARS-CoV-2 RNA from unextracted samples. We identify the optimal conditions to allow RPA-based amplification and Cas13-based detection to occur in a single step, simplifying assay preparation and reducing run-time. We improve HUDSON to rapidly inactivate viruses in nasopharyngeal swabs and saliva in 10 min. SHINE’s results can be visualized with an in-tube fluorescent readout — reducing contamination risk as amplification reaction tubes remain sealed — and interpreted by a companion smartphone application. We validate SHINE on 50 nasopharyngeal patient samples, demonstrating 90% sensitivity and 100% specificity compared to RT-qPCR with a sample-to-answer time of 50 min. SHINE has the potential to be used outside of hospitals and clinical laboratories, greatly enhancing diagnostic capabilities.
The COVID-19 pandemic has highlighted the need for user-friendly diagnostic techniques. Here, the authors present SHINE, a streamlined and optimised Cas13-based method with accompanying smartphone app for visual diagnosis.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 38/88
/ 45
/ 45/90
/ Betacoronavirus - isolation & purification
/ Coronavirus Infections - diagnosis
/ Coronavirus Infections - virology
/ COVID-19
/ CRISPR-Associated Proteins - metabolism
/ Humanities and Social Sciences
/ Humans
/ Molecular Diagnostic Techniques - methods
/ Pneumonia, Viral - diagnosis
/ RNA
/ Saliva
/ Science
/ Severe acute respiratory syndrome coronavirus 2
/ Tubes
