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Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs
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Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs
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Journal Article
Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs
2017
Overview
Anaphylactoid reactions are common clinical acute adverse drug reactions that can exacerbate a patient’s condition and produce effects that may become life-threatening. Therefore, it is important to establish a novel method to evaluate drugs for anaphylactoid reactions. In this study, we developed a sensitive and rapid method to detect histamine release from LAD2 cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and constructed a relative release index based on various release curve parameters, including allergen release time and sudden change rate, to evaluate the potential and strength of allergen-induced anaphylactoid reactions. This LAD2 release model was used to evaluate anaphylactoid reactions induced by ciprofloxacin, norfloxacin, lomefloxacin, moxifloxacin, and baicalin. The results positively correlated with those obtained with an Evans blue ear test and negatively correlated with the Ca
2+
influx EC
50
. In summary, the current study established a novel
in vitro
method to analyze the properties of histamine release from LAD2 cells and characterize the sensitization and strength of sensitization of drugs or components that may induce anaphylactoid reactions.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 631/250
/ 692/699
/ 82
/ 82/16
/ 82/58
/ 96
/ Anaphylaxis - chemically induced
/ Animals
/ Anti-Infective Agents - adverse effects
/ Baicalin
/ Cations, Divalent - metabolism
/ Chromatography, Liquid - methods
/ Humanities and Social Sciences
/ Humans
/ Male
/ Nerve Tissue Proteins - genetics
/ Nerve Tissue Proteins - metabolism
/ Receptors, G-Protein-Coupled - genetics
/ Receptors, G-Protein-Coupled - metabolism
/ Receptors, Neuropeptide - genetics
/ Receptors, Neuropeptide - metabolism
/ Science
