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Aerobic biogenesis of selenium nanoparticles by Enterobacter cloacae Z0206 as a consequence of fumarate reductase mediated selenite reduction
by
Xiao, Xiao
, Cheng, Yuanzhi
, Wang, Yizhen
, Li, Xiaoxiao
, Wang, Fengqin
, Song, Deguang
, Lu, Zeqing
in
140/146
/ 38/79
/ 631/326/171/1818
/ 631/326/2522
/ 631/326/88
/ Aerobic conditions
/ Aerobiosis
/ Bioreactors
/ Biosynthesis
/ Enterobacter cloacae
/ Enterobacter cloacae - enzymology
/ Enterobacter cloacae - metabolism
/ Glutathione
/ Humanities and Social Sciences
/ multidisciplinary
/ Nanoparticles
/ Nanoparticles - chemistry
/ Oxidation-Reduction
/ Reductase
/ Science
/ Science (multidisciplinary)
/ Selenium
/ Selenium - chemistry
/ Sodium
/ Sodium selenite
/ Sodium Selenite - chemistry
/ Sodium Selenite - metabolism
/ Succinate Dehydrogenase
2017
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Aerobic biogenesis of selenium nanoparticles by Enterobacter cloacae Z0206 as a consequence of fumarate reductase mediated selenite reduction
by
Xiao, Xiao
, Cheng, Yuanzhi
, Wang, Yizhen
, Li, Xiaoxiao
, Wang, Fengqin
, Song, Deguang
, Lu, Zeqing
in
140/146
/ 38/79
/ 631/326/171/1818
/ 631/326/2522
/ 631/326/88
/ Aerobic conditions
/ Aerobiosis
/ Bioreactors
/ Biosynthesis
/ Enterobacter cloacae
/ Enterobacter cloacae - enzymology
/ Enterobacter cloacae - metabolism
/ Glutathione
/ Humanities and Social Sciences
/ multidisciplinary
/ Nanoparticles
/ Nanoparticles - chemistry
/ Oxidation-Reduction
/ Reductase
/ Science
/ Science (multidisciplinary)
/ Selenium
/ Selenium - chemistry
/ Sodium
/ Sodium selenite
/ Sodium Selenite - chemistry
/ Sodium Selenite - metabolism
/ Succinate Dehydrogenase
2017
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Aerobic biogenesis of selenium nanoparticles by Enterobacter cloacae Z0206 as a consequence of fumarate reductase mediated selenite reduction
by
Xiao, Xiao
, Cheng, Yuanzhi
, Wang, Yizhen
, Li, Xiaoxiao
, Wang, Fengqin
, Song, Deguang
, Lu, Zeqing
in
140/146
/ 38/79
/ 631/326/171/1818
/ 631/326/2522
/ 631/326/88
/ Aerobic conditions
/ Aerobiosis
/ Bioreactors
/ Biosynthesis
/ Enterobacter cloacae
/ Enterobacter cloacae - enzymology
/ Enterobacter cloacae - metabolism
/ Glutathione
/ Humanities and Social Sciences
/ multidisciplinary
/ Nanoparticles
/ Nanoparticles - chemistry
/ Oxidation-Reduction
/ Reductase
/ Science
/ Science (multidisciplinary)
/ Selenium
/ Selenium - chemistry
/ Sodium
/ Sodium selenite
/ Sodium Selenite - chemistry
/ Sodium Selenite - metabolism
/ Succinate Dehydrogenase
2017
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Aerobic biogenesis of selenium nanoparticles by Enterobacter cloacae Z0206 as a consequence of fumarate reductase mediated selenite reduction
Journal Article
Aerobic biogenesis of selenium nanoparticles by Enterobacter cloacae Z0206 as a consequence of fumarate reductase mediated selenite reduction
2017
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Overview
In the present study, we examined the ability of
Enterobacter cloacae
Z0206 to reduce toxic sodium selenite and mechanism of this process.
E. cloacae
Z0206 was found to completely reduce up to 10 mM selenite to elemental selenium (Se°) and form selenium nanoparticles (SeNPs) under aerobic conditions. The selenite reducing effector of
E. cloacae
Z0206 cell was to be a membrane-localized enzyme. iTRAQ proteomic analysis revealed that selenite induced a significant increase in the expression of fumarate reductase. Furthermore, the addition of fumarate to the broth and knockout of
fumarate reductase
(
frd
) both significantly decreased the selenite reduction rate, which revealed a previously unrecognized role of
E. cloacae
Z0206 fumarate reductase in selenite reduction. In contrast, glutathione-mediated Painter-type reactions were not the main pathway of selenite reducing. In conclusion,
E. cloacae
Z0206 effectively reduced selenite to Se° using fumarate reductase and formed SeNPs; this capability may be employed to develop a bioreactor for treating Se pollution and for the biosynthesis of SeNPs in the future.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
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