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The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
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The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
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Journal Article
The live cell DNA stain SiR-Hoechst induces DNA damage responses and impairs cell cycle progression
2018
Overview
SiR-Hoechst (SiR-DNA) is a far-red fluorescent DNA probe being used widely for time-lapse imaging of living cells that is reported to be minimally toxic at concentrations as high as 10–25 µM. However, measuring nuclear import of Cyclin B1, inhibition of mitotic entry, and the induction of γH2AX foci in cultured human cells reveals that SiR-Hoechst induces DNA damage responses and G2 arrest at concentrations well below 1 µM. SiR-Hoechst is useful for live cell imaging, but it should be used with caution and at the lowest practicable concentration.
Publisher
Nature Publishing Group UK,Nature Publishing Group
