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Bright and stable monomeric green fluorescent protein derived from StayGold

by Papadaki, Stavrini , Drobizhev, Mikhail , Subach, Oksana M. , Zhang, Hanbin , Vlaskina, Anna V. , Subach, Fedor V. , Perfilov, Maxim M. , Samygina, Valeriya R. , Gabdulkhakov, Azat , Kuzmicheva, Tatyana P. , Chen, Liangyi , Zhang, Wenhao , Qin, Wenming , Lesnov, Gleb D. , Borshchevskiy, Valentin , Nikolaeva, Alena Yu , Gavrikov, Alexey S. , Piatkevich, Kiryl D. , Mishin, Alexander S. , Ye, Xianxin

in 631/1647/245 / 631/1647/245/2225 / Animals / Bioinformatics / Biological Microscopy / Biological Techniques / Biomedical and Life Sciences / Biomedical Engineering/Biotechnology / Fluorescence / Fluorescent Dyes / Green fluorescent protein / Green Fluorescent Proteins - metabolism / Image resolution / Labeling / Life Sciences / Luminescent Proteins - metabolism / Mice / Microscopy / Proteins / Proteomics / Structural proteins

2024

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Bright and stable monomeric green fluorescent protein derived from StayGold

2024

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2024

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Journal Article

Bright and stable monomeric green fluorescent protein derived from StayGold

Papadaki, Stavrini,

Drobizhev, Mikhail,

Subach, Oksana M.,

Zhang, Hanbin,

Vlaskina, Anna V.,

Subach, Fedor V.,

Perfilov, Maxim M.,

Samygina, Valeriya R.,

Gabdulkhakov, Azat,

Kuzmicheva, Tatyana P.,

Chen, Liangyi,

Zhang, Wenhao,

Qin, Wenming,

Lesnov, Gleb D.,

Borshchevskiy, Valentin,

Nikolaeva, Alena Yu,

Gavrikov, Alexey S.,

Piatkevich, Kiryl D.,

Mishin, Alexander S.,

Ye, Xianxin

2024

Overview

The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application as a fluorescent tag for some proteins. Here, we report the development and crystal structures of a monomeric variant of StayGold, named mBaoJin, which preserves the beneficial properties of its precursor, while serving as a tag for structural proteins and membranes. Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright and photostable fluorescent protein, exhibiting rapid maturation and high pH/chemical stability. mBaoJin was also demonstrated for super-resolution, long-term live-cell imaging and expansion microscopy. We further showed the applicability of mBaoJin for neuronal labeling in model organisms, including Caenorhabditis elegans and mice. mBaoJin is a monomeric derivative of the bright and photostable green fluorescent protein StayGold. mBaoJin offers favorable photophysical properties for use in diverse protein tagging and subcellular labeling applications.

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Publisher

Nature Publishing Group US,Nature Publishing Group

Subject

631/1647/245

/ 631/1647/245/2225

/ Animals

/ Bioinformatics

/ Biological Microscopy

/ Biological Techniques

/ Biomedical and Life Sciences