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An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
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An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
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Journal Article
An extended interaction site determines binding between AP180 and AP2 in clathrin mediated endocytosis
2024
Overview
The early phases of clathrin mediated endocytosis are organized through a highly complex interaction network mediated by clathrin associated sorting proteins (CLASPs) that comprise long intrinsically disordered regions (IDRs). AP180 is a CLASP exclusively expressed in neurons and comprises a long IDR of around 600 residues, whose function remains partially elusive. Using NMR spectroscopy, we discovered an extended and strong interaction site within AP180 with the major adaptor protein AP2, and describe its binding dynamics at atomic resolution. We find that the 70 residue-long site determines the overall interaction between AP180 and AP2 in a dynamic equilibrium between its bound and unbound states, while weaker binding sites contribute to the overall affinity at much higher concentrations of AP2. Our data suggest that this particular interaction site might play a central role in recruitment of adaptors to the clathrin coated pit, whereas more transient and promiscuous interactions allow reshaping of the interaction network until cargo uptake inside a coated vesicle.
The initial stages of clathrin mediated endocytosis are mediated by clathrin associated sorting proteins (CLASPs) encompassing long IDRs. Here the authors characterise the interaction of CLASP AP180 with the major adaptor protein AP2 using NMR.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 101/6
/ 82/80
/ 82/83
/ Adapters
/ Adaptor Protein Complex 2 - metabolism
/ Animals
/ Clathrin
/ Clathrin-Coated Vesicles - metabolism
/ Humanities and Social Sciences
/ Humans
/ Intrinsically Disordered Proteins - chemistry
/ Intrinsically Disordered Proteins - genetics
/ Intrinsically Disordered Proteins - metabolism
/ Magnetic Resonance Spectroscopy
/ Monomeric Clathrin Assembly Proteins - genetics
/ Monomeric Clathrin Assembly Proteins - metabolism
/ NMR
/ Proteins
/ Residues
/ Science
