Journal Article

Microplastic diversity increases the abundance of antibiotic resistance genes in soil

Wang, Yi-Fei, Fu, Yan-Mei, Rillig, Matthias C., Zhu, Yong-Guan, Zhu, Dong, Zhang, Tian-Lun, Liu, Yan-Jie, Cui, Hui-Ling, Qiao, Min, Xu, Jia-Yang

2024

Overview

The impact of microplastics on antibiotic resistance has attracted widespread attention. However, previous studies primarily focused on the effects of individual microplastics. In reality, diverse microplastic types accumulate in soil, and it remains less well studied whether microplastic diversity (i.e., variations in color, shape or polymer type) can be an important driver of increased antibiotic resistance gene (ARG) abundance. Here, we employed microcosm studies to investigate the effects of microplastic diversity on soil ARG dynamics through metagenomic analysis. Additionally, we evaluated the associated potential health risks by profiling virulence factor genes (VFGs) and mobile genetic elements (MGEs). Our findings reveal that as microplastic diversity increases, there is a corresponding rise in the abundance of soil ARGs, VFGs and MGEs. We further identified microbial adaptive strategies involving genes (changed genetic diversity), community (increased specific microbes), and functions (enriched metabolic pathways) that correlate with increased ARG abundance and may thus contribute to ARG dissemination. Additional global change factors, including fungicide application and plant diversity reduction, also contributed to elevated ARG abundance. Our findings suggest that, in addition to considering contamination levels, it is crucial to monitor microplastic diversity in ecosystems due to their potential role in driving the dissemination of antibiotic resistance through multiple pathways. The effects of microplastics (MPs) on soil microbial communities and antimicrobial resistance genes are not well understood. Here, the authors used microcosm studies to show that MP diversity, and partially fungicide treatment and reduced plant diversity, correlate with higher levels of ARG and related genetic factors.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 38/23 / 631/326/171 / 631/326/171/1818 / 631/326/22/1434 / 704/172/4081 / Abundance / Anti-Bacterial Agents - pharmacology / Antibiotic resistance / Antibiotics / Antimicrobial resistance / Bacteria - classification / Bacteria - drug effects / Bacteria - genetics / Drug resistance / Drug Resistance, Microbial - genetics / Fungicides / Genes / Genes, Bacterial / Genetic diversity / Genetic factors / Genetic Variation / Health risks / Humanities and Social Sciences / Impact resistance / Interspersed Repetitive Sequences - genetics / Metabolic pathways / Metagenome - genetics / Metagenomics / Microbial activity / Microcosms / Microorganisms / Microplastics / multidisciplinary / Plant diversity / Plastic debris / Plastic pollution / Polymers / Science / Science (multidisciplinary) / Soil - chemistry / Soil analysis / Soil investigations / Soil Microbiology / Soil Pollutants / Soil resistance / Soils / Virulence factors / Virulence Factors - genetics