Journal Article

Unraveling the complex interplay between obesity and vitamin D metabolism

AlMenhali, Asma, Munawar, Nayla, Yasin, Javed, Gariballa, Salah, Alzohily, Bashar, Shah, Iltaf

2024

Overview

Vitamin D deficiency and obesity are a worldwide health issue. Obesity refers to the accumulation of excessive fats in the body which could lead to the development of diseases. Obese people have low vitamin D levels for several reasons including larger volume of distribution, vitamin D tightly bound in fatty tissues, reduced absorption, and diets with low vitamin D. Accurately measuring vitamin D metabolites is challenging. The Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry (UHPLC-MS/MS) method was developed and validated for the analysis of vitamin D metabolites in the serum. Blood samples were collected from 452 subjects which consisted of baseline (vitamin D deficient obese subjects), follow-up (supplemented obese subjects), and healthy volunteers. The vitamin D metabolites were separated adequately by the developed UHPLC-MS/MS method. Moreover, the validation criteria for the method were within an acceptable range. The baseline, follow-up and even healthy volunteers were deficient in 25OHD3 and 25OHD2. The baseline and healthy subjects had comparable concentration of vitamin D2 and D3. However, healthy subjects had a higher concentration of 25OHD and its epimer compared to the baseline subjects. The vitamin D3 was increased significantly in the follow- up subjects; therefore, the 25OHD3 was increased significantly compared to the baseline as well; however, the increase was insufficient to achieve the optimal range. The UHPLC-MS/MS method test was applied successfully on estimation of vitamin D metabolites in subjects. This study indicates the significance of taking into account the metabolic and storage effects when evaluating the vitamin D status in obese subjects.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/1647 / 639/638 / 692/53 / 692/700 / Epimers of vitamin D / High-performance liquid chromatography / Humanities and Social Sciences / Liquid chromatography / Mass spectrometry / Mass spectroscopy / Metabolites / multidisciplinary / Nutrient deficiency / Obese subjects vitamin D deficiency / Obesity / Science / Science (multidisciplinary) / UHPLC-MS/MS / Vitamin D / Vitamin D blood measurement test / Vitamin D metabolites / Vitamin D2 / Vitamin D3 / Vitamin deficiency