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Single-cell brain organoid screening identifies developmental defects in autism
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Journal Article
Single-cell brain organoid screening identifies developmental defects in autism
2023
Overview
The development of the human brain involves unique processes (not observed in many other species) that can contribute to neurodevelopmental disorders
1
,
2
,
3
–
4
. Cerebral organoids enable the study of neurodevelopmental disorders in a human context. We have developed the CRISPR–human organoids–single-cell RNA sequencing (CHOOSE) system, which uses verified pairs of guide RNAs, inducible CRISPR–Cas9-based genetic disruption and single-cell transcriptomics for pooled loss-of-function screening in mosaic organoids. Here we show that perturbation of 36 high-risk autism spectrum disorder genes related to transcriptional regulation uncovers their effects on cell fate determination. We find that dorsal intermediate progenitors, ventral progenitors and upper-layer excitatory neurons are among the most vulnerable cell types. We construct a developmental gene regulatory network of cerebral organoids from single-cell transcriptomes and chromatin modalities and identify autism spectrum disorder-associated and perturbation-enriched regulatory modules. Perturbing members of the BRG1/BRM-associated factor (BAF) chromatin remodelling complex leads to enrichment of ventral telencephalon progenitors. Specifically, mutating the BAF subunit
ARID1B
affects the fate transition of progenitors to oligodendrocyte and interneuron precursor cells, a phenotype that we confirmed in patient-specific induced pluripotent stem cell-derived organoids. Our study paves the way for high-throughput phenotypic characterization of disease susceptibility genes in organoid models with cell state, molecular pathway and gene regulatory network readouts.
We develop a high-throughput CRISPR screening system in cerebral organoids and identify vulnerable cell types and gene regulatory networks associated with autism spectrum disorder from single-cell transcriptomes and chromatin modalities.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/107
/ 13/31
/ 13/44
/ 13/51
/ 14/63
/ 38/39
/ 45/47
/ Autism
/ Autism Spectrum Disorder - complications
/ Autism Spectrum Disorder - genetics
/ Autism Spectrum Disorder - pathology
/ Autistic Disorder - complications
/ Autistic Disorder - genetics
/ Autistic Disorder - pathology
/ Brain
/ Cloning
/ CRISPR
/ CRISPR-Associated Protein 9 - metabolism
/ Developmental Disabilities - complications
/ Developmental Disabilities - genetics
/ Developmental Disabilities - pathology
/ Genes
/ Humanities and Social Sciences
/ Humans
/ Neurodevelopmental disorders
/ RNA, Guide, CRISPR-Cas Systems
/ Science
