Journal Article

Comprehensive landscape of neutralizing antibody and cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF primary vaccination in adults

Reis, Laise Rodrigues, Martins-Filho, Olindo Assis, Abdala-Torres, Thais, de Lima, Sheila Maria Barbosa, Peruhype-Magalhães, Vanessa, de Noronha, Tatiana Guimarães, Lemos, Jandira Aparecida Campos, Maecker, Holden Terry, Domingues, Carla Magda Allan Santos, Camacho, Luiz Antônio Bastos, do Valle Antonelli, Lis Ribeiro, Duault, Caroline, de Lourdes de Sousa Maia, Maria, Almeida, Gregório Guilherme, Costa-Rocha, Ismael Artur, Araújo, Márcio Sobreira Silva, Coelho-dos-Reis, Jordana Grazziela, Schwarcz, Waleska Dias, Rosenberg-Hasson, Yael, Spezialli, Elaine, Fernandes, Eder Gatti, Chieppe, Alexandre, Fantinato, Francieli Fontana Sutile Tardetti, da Silva-Pereira, Rosiane Aparecida, Teixeira-Carvalho, Andréa, Campi-Azevedo, Ana Carolina, de Souza Azevedo, Adriana, Lemos, Maria Cristina Ferreira

2024

Overview

The present study aimed at evaluating the YF-specific neutralizing antibody profile besides a multiparametric analysis of phenotypic/functional features of cell-mediated response elicited by the 1/5 fractional dose of 17DD-YF vaccine, administered as a single subcutaneous injection. The immunological parameters of each volunteer was monitored at two time points, referred as: before (Day 0) [Non-Vaccinated, NV (D0) ] and after vaccination (Day 30–45) [Primary Vaccinees, PV (D30–45) ]. Data demonstrated high levels of neutralizing antibodies for PV (D30–45) leading to a seropositivity rate of 93%. A broad increase of systemic soluble mediators with a mixed profile was also observed for PV (D30–45) , with IFN-γ and TNF-α presenting the highest baseline fold changes. Integrative network mapping of soluble mediators showed increased correlation numbers in PV (D30–45) as compared to NV (D0) (532 vs 398). Moreover, PV (D30–45) exhibited increased levels of Terminal Effector (CD45RA + CCR7 − ) CD4 + and CD8 + T-cells and Non-Classical memory B-cells (IgD + CD27 + ). Dimensionality reduction of Mass Cytometry data further support these findings. A polyfunctional cytokine profile (TNF-α/IFN-γ/IL-10/IL-17/IL-2) of T and B-cells was observed upon in vitro antigen recall. Mapping and kinetics timeline of soluble mediator signatures for PV (D30–45) further confirmed the polyfunctional profile upon long-term in vitro culture, mediated by increased levels of IFN-γ and TNF-α along with decreased production of IL-10. These findings suggest novel insights of correlates of protection elicited by the 1/5 fractional dose of 17DD-YF vaccine.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 17DD vaccine / 631/250/127 / 631/250/1619 / 631/250/2152 / 631/250/590 / 631/250/590/1867 / 631/250/98 / 692/53 / Adult / Adults / Antibodies, Neutralizing / Antibodies, Viral / CD27 antigen / CD4 antigen / CD45RA antigen / CD8 antigen / CD8-Positive T-Lymphocytes / Cell culture / Cellular memory / Cytometry / Fractional dose / Humanities and Social Sciences / Humans / Immunization / Immunoglobulin D / Immunological memory / Interleukin 10 / Lymphocytes B / Lymphocytes T / Mapping / Memory cells / multidisciplinary / Neutralizing antibodies / Science / Science (multidisciplinary) / Tumor Necrosis Factor-alpha / Tumor necrosis factor-α / Vaccination / Vaccines / Yellow Fever / Yellow Fever Vaccine / γ-Interferon