Asset Details
Do you wish to reserve the book?
Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes
Do you wish to request the book?
Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes
2024
Overview
Although genome-wide polycistronic transcription places major emphasis on post-transcriptional controls in trypanosomatids, messenger RNA
cis
-regulatory untranslated regions (UTRs) have remained largely uncharacterised. Here, we describe a genome-scale massive parallel reporter assay coupled with 3’-UTR-seq profiling in the African trypanosome and identify thousands of regulatory UTRs. Increased translation efficiency was associated with dosage of adenine-rich poly-purine tracts (pPuTs). An independent assessment of native UTRs using machine learning based predictions confirmed the robust correspondence between pPuTs and positive control, as did an assessment of synthetic UTRs. Those 3’-UTRs associated with upregulated expression in bloodstream-stage cells were also enriched in uracil-rich poly-pyrimidine tracts, suggesting a mechanism for developmental activation through pPuT ‘unmasking’. Thus, we describe a
cis
-regulatory UTR sequence ‘code’ that underpins gene expression control in the context of a constitutively transcribed genome. We conclude that thousands of UTRs post-transcriptionally reprogram gene expression profiles in trypanosomes.
Cells that transcribe almost ‘everything everywhere all at once’ rely upon alternative expression controls. Here the authors employ massive parallel reporter assays and 3’UTR-seq to profile regulatory untranslated regions (UTRs) in African trypanosome.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
