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Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
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Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
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Journal Article
Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
2023
Overview
Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this issue by designing fusions of
Cas9
and guide RNA transcripts to tRNA-like sequence motifs that move RNAs from transgenic rootstocks to grafted wild-type shoots (scions) and achieve heritable gene editing, as demonstrated in wild-type
Arabidopsis thaliana
and
Brassica rapa
. The graft-mobile gene editing system enables the production of transgene-free offspring in one generation without the need for transgene elimination, culture recovery and selection, or use of viral editing vectors. We anticipate that using graft-mobile editing systems for transgene-free plant production may be applied to a wide range of breeding programs and crop plants.
Gene-edited plants free of CRISPR-associated sequences are generated by grafting.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
