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Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein

by Priola, Suzette A , Onwubiko, Henry A , Atarashi, Ryuichiro , Hughson, Andrew G , Caughey, Byron , Sim, Valerie L , Moore, Roger A , Dorward, David W

in Animals / Bioinformatics / Biological assay / Biological Microscopy / Biological Techniques / Biomedical and Life Sciences / Biomedical Engineering/Biotechnology / Blotting, Western / Brain / Cerebrospinal Fluid / Cricetinae / Electrophoresis, Polyacrylamide Gel / Genetic recombination / Hydrolysis / Life Sciences / Methods / Mice / Microscopy, Electron / Physiological aspects / Polymerization / Prions / Proteomics / PrPSc Proteins - analysis / Recombinant Proteins - analysis / Research methodology / Sensitivity and Specificity / Spectrophotometry, Ultraviolet

2007

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Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein

by Priola, Suzette A , Onwubiko, Henry A , Atarashi, Ryuichiro , Hughson, Andrew G , Caughey, Byron , Sim, Valerie L , Moore, Roger A , Dorward, David W

2007

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Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein

by Priola, Suzette A , Onwubiko, Henry A , Atarashi, Ryuichiro , Hughson, Andrew G , Caughey, Byron , Sim, Valerie L , Moore, Roger A , Dorward, David W

2007

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Journal Article

Ultrasensitive detection of scrapie prion protein using seeded conversion of recombinant prion protein

Priola, Suzette A,

Onwubiko, Henry A,

Atarashi, Ryuichiro,

Hughson, Andrew G,

Caughey, Byron,

Sim, Valerie L,

Moore, Roger A,

Dorward, David W

2007

Overview

The scrapie prion protein isoform, PrP Sc , is a prion-associated marker that seeds the conformational conversion and polymerization of normal protease-sensitive prion protein (PrP-sen). This seeding activity allows ultrasensitive detection of PrP Sc using cyclical sonicated amplification (PMCA) reactions and brain homogenate as a source of PrP-sen. Here we describe a much faster seeded polymerization method (rPrP-PMCA) which detects ≥50 ag of hamster PrP Sc (≈0.003 lethal dose) within 2–3 d. This technique uses recombinant hamster PrP-sen, which, unlike brain-derived PrP-sen, can be easily concentrated, mutated and synthetically tagged. We generated protease-resistant recombinant PrP fibrils that differed from spontaneously initiated fibrils in their proteolytic susceptibility and by their infrared spectra. This assay could discriminate between scrapie-infected and uninfected hamsters using 2-μl aliquots of cerebral spinal fluid. This method should facilitate the development of rapid, ultrasensitive prion assays and diagnostic tests, in addition to aiding fundamental studies of structure and mechanism of PrP Sc formation.

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Publisher

Nature Publishing Group US,Nature Publishing Group

Subject

Animals

/ Bioinformatics

/ Biological assay

/ Biological Microscopy

/ Biological Techniques

/ Biomedical and Life Sciences

/ Biomedical Engineering/Biotechnology