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An image analysis toolbox for high-throughput C. elegans assays
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An image analysis toolbox for high-throughput C. elegans assays
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Journal Article
An image analysis toolbox for high-throughput C. elegans assays
2012
Overview
The freely available WormToolbox enables high-throughput image analysis of a variety of phenotypes of
Caenorhabditis elegans
in liquid culture and should prove useful for image-based screens.
We present a toolbox for high-throughput screening of image-based
Caenorhabditis elegans
phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available through the open-source CellProfiler project and enables objective scoring of whole-worm high-throughput image-based assays of
C. elegans
for the study of diverse biological pathways that are relevant to human disease.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Analysis
/ Animals
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Caenorhabditis elegans - cytology
/ Computerized Image Processing
/ High-Throughput Screening Assays - instrumentation
/ High-Throughput Screening Assays - methods
/ Image Processing, Computer-Assisted - instrumentation
/ Image Processing, Computer-Assisted - methods
/ Methods
/ Microscopy, Fluorescence - instrumentation
/ Microscopy, Fluorescence - methods
/ Pattern Recognition, Automated - methods
/ Software
/ Worms
