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An improved surface passivation method for single-molecule studies
by
Ha, Taekjip
, Singh, Digvijay
, Kim, Hajin
, Abeysirigunawardena, Sanjaya C
, Hua, Boyang
, Han, Kyu Young
, Aggarwal, Vasudha
, Woodson, Sarah A
, Zhou, Ruobo
, Shi, Xinghua
, Jain, Ankur
in
Deoxyribonucleic acid
/ Diagnostic imaging
/ DNA
/ DNA-Directed DNA Polymerase - metabolism
/ Fluorescence Resonance Energy Transfer - methods
/ Humans
/ Innovations
/ Molecular biology
/ Polyethylene Glycols - chemistry
/ Polyethylene Glycols - metabolism
/ Polysorbates - chemistry
/ Polysorbates - metabolism
/ Proliferating Cell Nuclear Antigen - metabolism
/ Properties
/ RNA-protein interactions
/ Silane
/ Silanes - chemistry
/ Silanes - metabolism
/ Studies
/ Surface Properties
/ Surfaces
/ Surfaces (Technology)
2014
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An improved surface passivation method for single-molecule studies
by
Ha, Taekjip
, Singh, Digvijay
, Kim, Hajin
, Abeysirigunawardena, Sanjaya C
, Hua, Boyang
, Han, Kyu Young
, Aggarwal, Vasudha
, Woodson, Sarah A
, Zhou, Ruobo
, Shi, Xinghua
, Jain, Ankur
in
Deoxyribonucleic acid
/ Diagnostic imaging
/ DNA
/ DNA-Directed DNA Polymerase - metabolism
/ Fluorescence Resonance Energy Transfer - methods
/ Humans
/ Innovations
/ Molecular biology
/ Polyethylene Glycols - chemistry
/ Polyethylene Glycols - metabolism
/ Polysorbates - chemistry
/ Polysorbates - metabolism
/ Proliferating Cell Nuclear Antigen - metabolism
/ Properties
/ RNA-protein interactions
/ Silane
/ Silanes - chemistry
/ Silanes - metabolism
/ Studies
/ Surface Properties
/ Surfaces
/ Surfaces (Technology)
2014
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An improved surface passivation method for single-molecule studies
by
Ha, Taekjip
, Singh, Digvijay
, Kim, Hajin
, Abeysirigunawardena, Sanjaya C
, Hua, Boyang
, Han, Kyu Young
, Aggarwal, Vasudha
, Woodson, Sarah A
, Zhou, Ruobo
, Shi, Xinghua
, Jain, Ankur
in
Deoxyribonucleic acid
/ Diagnostic imaging
/ DNA
/ DNA-Directed DNA Polymerase - metabolism
/ Fluorescence Resonance Energy Transfer - methods
/ Humans
/ Innovations
/ Molecular biology
/ Polyethylene Glycols - chemistry
/ Polyethylene Glycols - metabolism
/ Polysorbates - chemistry
/ Polysorbates - metabolism
/ Proliferating Cell Nuclear Antigen - metabolism
/ Properties
/ RNA-protein interactions
/ Silane
/ Silanes - chemistry
/ Silanes - metabolism
/ Studies
/ Surface Properties
/ Surfaces
/ Surfaces (Technology)
2014
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An improved surface passivation method for single-molecule studies
Journal Article
An improved surface passivation method for single-molecule studies
2014
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Overview
We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly(ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
Publisher
Nature Publishing Group
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