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Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress
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Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress
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Journal Article
Sulforaphane prevents rat cardiomyocytes from hypoxia/reoxygenation injury in vitro via activating SIRT1 and subsequently inhibiting ER stress
2016
Overview
Aim： Sulforaphane （SFN）, a natural dietary isothiocyanate, is found to exert beneficial effects for cardiovascular diseases. This study aimed to investigate the mechanisms underlying the protective effects of SFN in a model of myocardial hypoxia/reoxygenation （H/R） inj u ry in vitro. Methods： Cultured neonatal rat cardiomyocytes pretreated with SFN were subjected to 3-h hypoxia followed by 3-h reoxygenation. Cell viability and apoptosis were detected. Caspase-3 activity and mitochondrial membrane potential （△ψm） was measured. The expression of ER stress-related apoptotic proteins were analyzed with Western blot analyses. Silent information regulator 1 （SIRT1） activity was determined with SIRT1 deacetylase fluorometric assay kit. Results： SFN （0.1-5 pmol/L） dose-dependently improved the viability of cardiomyocytes, diminished apoptotic cells and suppressed caspase-3 activity. Meanwhile, SFN significantly alleviated the damage of △ψm and decreased the expression of ER stress-related apoptosis proteins （GRP78, CHOP and caspase-12）, elevating the expression of SIRT1 and Bcl-2/Bax ratio in the cardiomyocytes. Co-treatment of the cardiomyocytes with the SIRTl-specific inhibitor Ex-527 （1 μmol/L） blocked the SFN-Jnduced cardioprotective effects.Conclusion： SFN prevents cardiomyocytes from H/R injury in vitro most likely via activating SIRT1 pathway and subsequently inhibiting the ER stress-dependent apoptosis.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Biomedical and Life Sciences
/ Cardiotonic Agents - pharmacology
/ Cell Survival - drug effects
/ Endoplasmic Reticulum Stress - drug effects
/ Isothiocyanates - pharmacology
/ Membrane Potential, Mitochondrial - drug effects
/ Myocardial Reperfusion Injury - metabolism
/ Myocardial Reperfusion Injury - pathology
/ Myocardial Reperfusion Injury - prevention & control
/ Myocytes, Cardiac - drug effects
/ Myocytes, Cardiac - metabolism
/ Myocytes, Cardiac - pathology
/ Original
/ Rats
/ Signal Transduction - drug effects
/ Vaccine
/ 体外培养
/ 内质网应激
/ 心肌细胞
/ 新生大鼠
/ 激活
/ 硫素
/ 缺氧/复氧损伤
/ 萝卜
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