Journal Article

Absolute direction in organelle movement

Gebhardt, Philipp, Schneider, Gaby, Meyer, Annika, Ernst, Theresa, Schleiff, Enrico, Plomer, Solveig

2024

Overview

In movement analysis, correlated random walk (CRW) models often use so‐called turning angles, which are measured relative to the previous movement direction. To segregate between different movement modes, hidden Markov models (HMMs) describe movements as piecewise stationary CRWs in which the distributions of turning angles and step sizes depend on the underlying state. This typically allows for the segregation of movement modes that show different movement speeds. We show that in some cases, it may be interesting to investigate absolute angles, that is, biased random walks (BRWs) instead of turning angles. In particular, while discrimination between states in the turning angle setting can only rely on movement speed, models with absolute angles can be used to discriminate between sections of different movement directions. A preprocessing algorithm is provided that enables the analysis of absolute angles in the existing R package moveHMM. In a data set of movements of cell organelles, models using not the turning angle but the absolute angle could capture interesting additional properties. Goodness‐of‐fit was increased for HMMs with absolute angles, and HMMs with absolute angles tended to choose a higher number of states, suggesting the existence and relevance of prominent directional changes in the present data set. These results suggest that models with absolute angles can provide important information in the analysis of movement patterns if the existence and frequency of directional changes is of biological importance. In movement analysis, models often use so‐called turning angles, which are measured relative to the previous movement direction. We use hidden Markov models (HMMs) to show that the analysis of absolute angles instead of turning angles can provide important additional information if the existence and frequency of directional changes is of biological relevance. In a data set of movements of cell organelles, goodness‐of‐fit was most often increased for HMMs with absolute angles, and these could therefore be used to investigate important additional properties, such as the switching of movement between periods of different movement directions.

• Publisher: John Wiley & Sons, Inc,John Wiley and Sons Inc,Wiley
• Subject: absolute angle / Algorithms / Bias / biased random walk / Biological effects / Cell culture / Cell migration / correlated random walk / Data analysis / Datasets / hidden Markov model / Markov chains / movement analysis / Organelles / Probability distribution / Random walk / turning angle