Journal Article

Biocompatibility evaluation of antibacterial Ti-Ag alloys with nanotubular coatings

Chen, Chen, You, Junhua, Chen, Shiyi, Zhang, Hangzhou, Lei, Zeming, Bai, Xizhuang, Tian, Ang, Liu, Xingwang, Li, Xi, Wu, Lin

2019

Overview

Implant-related infection is a major problem postsurgery. As an alternative to a localized antibiotic release system, we used Ag to fabricate Ti-Ag alloys with nanotubular coatings (TiAg-NTs). Ag has excellent antibacterial properties, but its biological toxicity is a concern. Therefore, we performed biological experiments both in vitro and in vivo to evaluate the biocompatibility of TiAg-NTs with different concentrations of Ag (1%, 2%, and 4%). For in vitro experiments, cytocompatibility, including cell attachment, viability, and proliferation, was tested, and genes and proteins related to osteogenic differentiation were also evaluated. For in vivo assays, the rat femoral condylar insertion model was used, and micro-computed tomography (micro-CT) and histological analysis were conducted to analyze bone formation around implants at 1, 2, and 4 weeks after surgery. Both in vitro and in vivo results indicate that Ti2%Ag-NT showed comparable cytocompatibility with commercially pure Ti (cp-Ti), and it could achieve good osseointegration with the surrounding bone tissue. We thus believe that Ti2%Ag-NT is a potential biomaterial for orthopedics.

• Publisher: Dove Medical Press Limited,Taylor & Francis Ltd,Dove Press,Dove Medical Press
• Subject: alloy / Alloys / Alloys - chemistry / Alloys - pharmacology / Animals / Anti-Bacterial Agents - pharmacology / Antibacterial agents / Antibiotics / Antineoplastic Agents - pharmacology / Bacterial Adhesion / Biocompatibility / Biological products / Biomedical materials / Biotechnology industry / Bone Neoplasms - drug therapy / Bone Neoplasms - pathology / Cell adhesion & migration / Cell Proliferation / Coated Materials, Biocompatible - chemistry / Coatings / Cytotoxicity / Degassing of metals / Experiments / Health aspects / Humans / Hydroxyapatite / Joint surgery / Laboratories / Male / Metals / Nanoparticles / nanotube / Nanotubes - chemistry / Original Research / Osteogenesis / Osteosarcoma - drug therapy / Osteosarcoma - pathology / Povidone / Proteins / Rats / Rats, Sprague-Dawley / Scientific equipment and supplies industry / Silver / Silver - chemistry / Specialty metals industry / Sports medicine / Surgery / Titanium - chemistry / Transplants & implants / Tumor Cells, Cultured