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An antibiotic selection marker for nematode transgenesis
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Journal Article
An antibiotic selection marker for nematode transgenesis
2010
Overview
A gene conferring neomycin resistance can be used for antibiotic selection in
C. elegans
and
C. briggsae
. This will permit easy maintenance of transgenic lines and facilitate single-copy insertion of transgenes. Also in this issue, a related paper reports nematode selection using puromycin.
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (
NeoR
) and shown that it could confer resistance to G-418 on both wild-type
Caenorhabditis elegans
and
C. briggsae
. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ Animals
/ Animals, Genetically Modified
/ Biomedical and Life Sciences
/ Biomedical Engineering/Biotechnology
/ Caenorhabditis - classification
/ Caenorhabditis - drug effects
/ Drug Resistance - drug effects
/ Drug resistance in microorganisms
/ Genomics
/ Nematoda
/ Selection, Genetic - drug effects
