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The peptide-binding motif of HLA-DR8 shares important structural features with other type 1 diabetes-associated alleles
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The peptide-binding motif of HLA-DR8 shares important structural features with other type 1 diabetes-associated alleles
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Journal Article
The peptide-binding motif of HLA-DR8 shares important structural features with other type 1 diabetes-associated alleles
2011
Overview
The objective of this study was to characterize the peptide-binding motif of the major histocompatibility complex (MHC) class II HLA-DR8 molecule included in the type 1 diabetes-associated haplotype DRB1
*
0801-DQA1
*
0401/DQB1
*
0402 (DR8-DQ4), and compare it with that of other diabetes-associated MHC class II alleles; DR8-bound peptides were eluted from an HLA-DR homozygous lymphoblastoid cell line. The repertoire was characterized by peptide sequencing using a LTQ ion trap mass spectrometer coupled to a multidimensional liquid chromatography system. After validation of the spectra identification, the definition of the HLA-DR8 peptide-binding motif was achieved from the analysis of 486 natural ligands, based on serial alignments of all possible HLA-DR-binding cores. The DR8 motif showed a strong similarity with the peptide-binding motifs of other MHC class II diabetes-associated alleles, HLA-DQ8 and H-2 I-A
g7
. Similar to HLA-DQ8 and H-2 I-A
g7
, HLA-DR8 preferentially binds peptides with an acidic residue at position P9 of the binding core, indicating that DR8 is the susceptibility component of the DR8-DQ4 haplotype. Indeed, some DR8 peptides were identical to peptides previously identified as DQ8- or I-A
g7
ligands, and several diabetes-specific peptides associated with DQ8 or I-A
g7
could theoretically bind to HLA-DR8. These data further strengthen the association of HLA-DR8 with type I diabetes.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Alleles
/ Analysis
/ Antigens
/ Biomedical and Life Sciences
/ Diabetes
/ Diabetes mellitus (insulin dependent)
/ Diabetes Mellitus, Type 1 - genetics
/ Genes
/ Histocompatibility antigen H-2
/ Histocompatibility antigen HLA
/ HLA histocompatibility antigens
/ HLA-DR Serological Subtypes - chemistry
/ HLA-DR Serological Subtypes - metabolism
/ Humans
/ Ligands
/ Major histocompatibility complex
/ Peptides
/ Proteins
