Journal Article

Outbreak of Neisseria meningitidis serogroup C outside the meningitis belt—Liberia, 2017: an epidemiological and laboratory investigation

Tamatai, George, Diaz, Maureen H, Paasewe, Thomas, Taweh, Fahn, Naiene, Jeremias, Wang, Xin, Bozio, Catherine H, Brima-Davis, Annette, Jetoh, Ralph, Larway, Lawrence, Stephen, Olayinka, Monger, Thomas, Kinkade, Carl, Allen, Denise, Gasasira, Alex, Konway, Youhn, Kerwillain, Garrison, Retchless, Adam C, Wilson, Himiede W, Chang, Arthur, Mahon, Barbara E, Yealue, Kwuakuan D M, Yarkeh, James, Gweh, Dedesco, Patel, Jaymin C, Clement, Peter, Stone, Mardia, Senneh, George, Asamoah Frimpong, Joseph, McNamara, Lucy A, Williams, Desmond, Schmink, Susanna E, Vera Walker, Yatta, Clark, Thomas A, Taha, Muhamed, Doedeh, Vivian, Smith, Samuel, George, Roseline N, Gorwor, Lawrence, Djingarey, Harouna M, Freeman, Maxwell, Christian, Kira, Ghartey, Emmanuel, Katawera, Victoria, Gbanya, Miatta, Fox, LeAnne M, Redd, John T, Potts, Caelin C, Perrocheau, Anne, Gwesa, Gulu, Bao, Umaru, Fallah, Mosoka P, Hardy, Patrick, Jenkins, Laurel T, Waller, Jessica L, Whaley, Melissa J, Schier, Joshua, Toe, Sylvester, Winchell, Jonas M, Dauda, George, Mahmoud, Nuha, George, Geraldine, Reed, Mulbah, Nagbe, Thomas, Amo-Addae, Maame, Vuong, Jeni, Koha

2018

Overview

On April 25, 2017, a cluster of unexplained illnesses and deaths associated with a funeral was reported in Sinoe County, Liberia. Molecular testing identified Neisseria meningitidis serogroup C (NmC) in specimens from patients. We describe the epidemiological investigation of this cluster and metagenomic characterisation of the outbreak strain. We collected epidemiological data from the field investigation and medical records review. Confirmed, probable, and suspected cases were defined on the basis of molecular testing and signs or symptoms of meningococcal disease. Metagenomic sequences from patient specimens were compared with 141 meningococcal isolate genomes to determine strain lineage. 28 meningococcal disease cases were identified, with dates of symptom onset from April 21 to April 30, 2017: 13 confirmed, three probable, and 12 suspected. 13 patients died. Six (21%) patients reported fever and 23 (82%) reported gastrointestinal symptoms. The attack rate for confirmed and probable cases among funeral attendees was 10%. Metagenomic sequences from six patient specimens were similar to a sequence type (ST) 10217 (clonal complex [CC] 10217) isolate genome from Niger, 2015. Multilocus sequencing identified five of seven alleles from one specimen that matched ST-9367, which is represented in the PubMLST database by one carriage isolate from Burkina Faso, in 2011, and belongs to CC10217. This outbreak featured high attack and case fatality rates. Clinical presentation was broadly consistent with previous meningococcal disease outbreaks, but predominance of gastrointestinal symptoms was unusual compared with previous African meningitis epidemics. The outbreak strain was genetically similar to NmC CC10217, which caused meningococcal disease outbreaks in Niger and Nigeria. CC10217 had previously been identified only in the African meningitis belt. US Global Health Security.

• Publisher: Elsevier Ltd,Elsevier Limited
• Subject: Adolescent / Adult / Aged / Aged, 80 and over / Child / Clusters / Contact Tracing / Deoxyribonucleic acid / Disease Outbreaks / DNA / Ebola virus / Epidemics / Epidemiology / Fatalities / Female / Fever / Field investigations / Gastrointestinal symptoms / Gene sequencing / Genomes / Genotype / Global health / Headaches / Humans / Illnesses / Infectious diseases / Liberia - epidemiology / Male / Medical laboratories / Medical records / Meningitis / Meningitis, Meningococcal - epidemiology / Meningitis, Meningococcal - microbiology / Meningitis, Meningococcal - mortality / Meningitis, Meningococcal - pathology / Meningococcal disease / Metagenomics / Middle Aged / Molecular Epidemiology / Multilocus Sequence Typing / Neisseria meningitidis / Neisseria meningitidis, Serogroup C - classification / Neisseria meningitidis, Serogroup C - genetics / Neisseria meningitidis, Serogroup C - isolation & purification / Nucleotide sequence / Outbreaks / Patients / Plasma / Public health / Survival Analysis / Urine / Vaccines / Vomiting / Young Adult