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A Y2H-seq approach defines the human protein methyltransferase interactome
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A Y2H-seq approach defines the human protein methyltransferase interactome
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Journal Article
A Y2H-seq approach defines the human protein methyltransferase interactome
2013
Overview
A high-sensitivity, high-quality yeast two-hybrid screen using short-read sequencing as its readout is used to map the interactome of human methyltransferases.
To accelerate high-density interactome mapping, we developed a yeast two-hybrid interaction screening approach involving short-read second-generation sequencing (Y2H-seq) with improved sensitivity and a quantitative scoring readout allowing rapid interaction validation. We applied Y2H-seq to investigate enzymes involved in protein methylation, a largely unexplored post-translational modification. The reported network of 523 interactions involving 22 methyltransferases or demethylases is comprehensively annotated and validated through coimmunoprecipitation experiments and defines previously undiscovered cellular roles of nonhistone protein methylation.
Publisher
Nature Publishing Group US,Nature Publishing Group
Subject
/ 631/553
/ Biomedical Engineering/Biotechnology
/ Enzymes
/ Gene Expression Regulation, Enzymologic - physiology
/ Humans
/ Identification and classification
/ Methyltransferases - genetics
/ Methyltransferases - metabolism
/ Post-translational modification
/ Protein Interaction Mapping - methods
/ Proteins
/ Sequence Analysis, DNA - methods
/ Two-Hybrid System Techniques
/ Yeasts
