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Isolation and Identification of Fusarium Wilt Pathogens from Bitter Gourd in Hainan Province
by
Ziji LIU Gongfu DU Jie ZHU Yan YANG
in
Agriculture
/ Biotypes
/ Crop diseases
/ Crops
/ Deoxyribonucleic acid
/ DNA
/ Fusarium
/ Genetic testing
/ Identification
/ JNK protein
/ Morphology
/ Pathogens
/ Phylogenetics
/ Physical characteristics
/ Plant diseases
/ Wilt
/ 分离鉴定
/ 枯萎病菌
/ 海南省
/ 特异性试验
/ 系统发育分析
/ 苦瓜枯萎病
/ 表现症状
/ 镰刀菌
2017
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Isolation and Identification of Fusarium Wilt Pathogens from Bitter Gourd in Hainan Province
by
Ziji LIU Gongfu DU Jie ZHU Yan YANG
in
Agriculture
/ Biotypes
/ Crop diseases
/ Crops
/ Deoxyribonucleic acid
/ DNA
/ Fusarium
/ Genetic testing
/ Identification
/ JNK protein
/ Morphology
/ Pathogens
/ Phylogenetics
/ Physical characteristics
/ Plant diseases
/ Wilt
/ 分离鉴定
/ 枯萎病菌
/ 海南省
/ 特异性试验
/ 系统发育分析
/ 苦瓜枯萎病
/ 表现症状
/ 镰刀菌
2017
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Isolation and Identification of Fusarium Wilt Pathogens from Bitter Gourd in Hainan Province
by
Ziji LIU Gongfu DU Jie ZHU Yan YANG
in
Agriculture
/ Biotypes
/ Crop diseases
/ Crops
/ Deoxyribonucleic acid
/ DNA
/ Fusarium
/ Genetic testing
/ Identification
/ JNK protein
/ Morphology
/ Pathogens
/ Phylogenetics
/ Physical characteristics
/ Plant diseases
/ Wilt
/ 分离鉴定
/ 枯萎病菌
/ 海南省
/ 特异性试验
/ 系统发育分析
/ 苦瓜枯萎病
/ 表现症状
/ 镰刀菌
2017
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Isolation and Identification of Fusarium Wilt Pathogens from Bitter Gourd in Hainan Province
Journal Article
Isolation and Identification of Fusarium Wilt Pathogens from Bitter Gourd in Hainan Province
2017
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Overview
In order to isolate and identify the pathogens causing Fusarium wilt in bitter gourd in Hainan Province, China, four bitter gourd plants exhibiting symptoms of Fusarium wilt were collected from Tunchang County, Hainan Province. Four pathogen strains (HNTC-01, HNTC-02, HNTC-03 and HNTC-04) were isolated from the four plants, and identified via morphological observation and molecular techniques. The results showed that HNTC--01, HNTC-03 and HNTC-04 were F. oxysporum, while HNTC-02 was F. graminearum. The tests on pathogenicity and host specificity showed that HNTC-01, HNTC-03 and HNTC-04 were F. oxysporum Schl. f. sp. momordicae. Phylogenetic analysis revealed that HNTC-01, HNTC-03, HNTC-04 were clustered together with 14 strains of F. oxysporum, and HNTC-02 was clustered together with six strains of F. graminearum. In addition, rDNA-ITS region can be used to distinguish different Fusarium species, but can not be used to distinguish different biotypes within a Fusarium species. This study could provide a scientific basis for the diagnosis and prevention of wilt in bitter gourd.
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