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Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster
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Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster
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Journal Article
Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster
2008
Overview
To enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy number plasmid, pBluescript SK(+). The production of EPA was remarkably enhanced yielding levels of up to 7.5% of the total fatty acid content in the recombinant E. coli strain by induction with IPTG, whereas the stimulation of EPA production was abolished by adding glucose into the culture medium, probably due to glucose repression acting on the promoter activity.
Publisher
Dordrecht : Springer Netherlands,Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Clusters
/ Culture
/ E coli
/ Eicosapentaenoic Acid - biosynthesis
/ Eicosapentaenoic Acid - genetics
/ Escherichia coli - metabolism
/ Fundamental and applied biological sciences. Psychology
/ Gas Chromatography-Mass Spectrometry
/ Genes
/ Genetics
/ Glucose
/ plasmids
/ Transferases (Other Substituted Phosphate Groups)
/ Transferases (Other Substituted Phosphate Groups) - genetics
