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Development of genetic markers in Yarrowia lipolytica
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Journal Article
Development of genetic markers in Yarrowia lipolytica
2024
Overview
The oleaginous yeast
Yarrowia lipolytica
represents a potential microbial cell factory for the recombinant production of various valuable products. Currently, the commonly used selection markers for transformation in
Y. lipolytica
are limited, and successive genetic manipulations are often restricted by the number of available selection markers. In our study, we developed a dominant marker,
dsdA
, which encodes a D-serine deaminase for genetic manipulation in
Y. lipolytica
. In
Y. lipolytica
, this marker confers the ability to use D-serine as a nitrogen source. In addition, the selection conditions of several infrequently used dominant markers including
bleoR
(zeocin resistance),
kan
MX (G418 resistance), and
guaB
(mycophenolic acid resistance) were also analyzed. Our results demonstrated that these selection markers can be used for the genetic manipulation of
Y. lipolytica
and their selection conditions were different for various strains. Ultimately, the selection markers tested here will be useful to expand the genetic toolbox of
Y. lipolytica
.
Key points
• The dsdA from Escherichia coli was developed as a dominant marker.
• The applicability of several resistance markers in Y. lipolytica was determined.
• We introduced the Cre/mutant lox system for marker recycling.
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
