Asset Details
Do you wish to reserve the book?
Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A
Do you wish to request the book?
Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Comparable T helper 1 (Th1) and CD8 T-cell immunity by targeting HIV gag p24 to CD8 dendritic cells within antibodies to Langerin, DEC205, and Clec9A
2011
Overview
Improved protein-based vaccines should facilitate the goal of effective vaccines against HIV and other pathogens. With respect to T cells, the efficiency of immunization, or \"immunogenicity,\" is improved by targeting vaccine proteins to maturing dendritic cells (DCs) within mAbs to DC receptors. Here, we compared the capacity of Langerin/CD207, DEC205/CD205, and Clec9A receptors, each expressed on the CD8⁺ DC subset in mice, to bring about immunization of microbial-specific T cells from the polyclonal repertoire, using HIV gag-p24 protein as an antigen. α-Langerin mAb targeted splenic CD8⁺ DCs selectively in vivo, whereas α-DEC205 and α-Clec9A mAbs targeted additional cell types. When the mAb heavy chains were engineered to express gag-p24, the α-Langerin, α-DEC205, and α-Clec9A fusion mAbs given along with a maturation stimulus induced comparable levels of gag-specific T helper 1 (Th1) and CD8⁺ T cells in BALB/c x C57BL/6 F1 mice. These immune T cells were more numerous than targeting the CD8⁻ DC subset with α-DCIR2-gag-p24. In an in vivo assay in which gag-primed T cells were used to report the early stages of T-cell responses, α-Langerin, α-DEC205, and α-Clec9A also mediated cross-presentation to primed CD8⁺ T cells if, in parallel to antigen uptake, the DCs were stimulated with α-CD40. α-Langerin, α-DEC205, and α-Clec9A targeting greatly enhanced T-cell immunization relative to nonbinding control mAb or nontargeted HIV gag-p24 protein. Therefore, when the appropriate subset of DCs is targeted with a vaccine protein, several different receptors expressed by that subset are able to initiate combined Th1 and CD8⁺ immunity.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ AIDS Vaccines - pharmacology
/ Animals
/ Antibodies, Monoclonal - immunology
/ Antibodies, Monoclonal - pharmacology
/ Antigens
/ Antigens, Surface - immunology
/ CD8-Positive T-Lymphocytes - immunology
/ Dendritic Cells - immunology
/ HIV
/ HIV Core Protein p24 - immunology
/ HIV Core Protein p24 - pharmacology
/ Human immunodeficiency virus
/ Immunity
/ Immunity, Cellular - drug effects
/ Immunity, Cellular - immunology
/ Lectins
/ Lectins, C-Type - immunology
/ Mannose-Binding Lectins - immunology
/ Mice
/ Minor Histocompatibility Antigens
/ Proteins
/ Receptors, Cell Surface - immunology
/ Receptors, Immunologic - immunology
/ Rodents
/ Spleen
/ Vaccines
