Journal Article

Functional CD47/signal regulatory protein alpha (SIRPα) interaction is required for optimal human T- and natural killer- (NK) cell homeostasis in vivo

de Geus, Sandra J, Pouw, Stephan M, Di Santo, James P, Legrand, Nicolas, Huntington, Nicholas D, Bakker, Arjen Q, Böhne, Martino, Schotte, Remko, Voordouw, Arie, Spits, Hergen, Strick-Marchand, Hélène, Nagasawa, Maho, Weijer, Kees

2011

Overview

The homeostatic control mechanisms regulating human leukocyte numbers are poorly understood. Here, we assessed the role of phagocytes in this process using human immune system (HIS) BALB/c Rag2⁻/⁻IL-2Rγc⁻/⁻ mice in which human leukocytes are generated from transplanted hematopoietic progenitor cells. Interactions between signal regulatory protein alpha (SIRPα; expressed on phagocytes) and CD47 (expressed on hematopoietic cells) negatively regulate phagocyte activity of macrophages and other phagocytic cells. We previously showed that B cells develop and survive robustly in HIS mice, whereas T and natural killer (NK) cells survive poorly. Because human CD47 does not interact with BALB/c mouse SIRPα, we introduced functional CD47/SIRPα interactions in HIS mice by transducing mouse CD47 into human progenitor cells. Here, we show that this procedure resulted in a dramatic and selective improvement of progenitor cell engraftment and human T- and NK-cell homeostasis in HIS mouse peripheral lymphoid organs. The amount of engrafted human B cells also increased but much less than that of T and NK cells, and total plasma IgM and IgG concentrations increased 68- and 35-fold, respectively. Whereas T cells exhibit an activated/memory phenotype in the absence of functional CD47/SIRPα interactions, human T cells accumulated as CD4⁺ or CD8⁺ single-positive, naive, resting T cells in the presence of functional CD47/SIRPα interactions. Thus, in addition to signals mediated by T cell receptor (TCR)/MHC and/or IL/IL receptor interactions, sensing of cell surface CD47 expression by phagocyte SIRPα is a critical determinant of T- and NK-cell homeostasis under steady-state conditions in vivo.

• Publisher: National Academy of Sciences,National Acad Sciences
• Subject: Animals / Antigens, Differentiation - metabolism / B lymphocytes / B-Lymphocytes - cytology / B-Lymphocytes - metabolism / Biological Sciences / CD4 antigen / CD47 Antigen - metabolism / CD8 antigen / Cell surface / Cells / DNA-Binding Proteins - deficiency / DNA-Binding Proteins - metabolism / Gene expression / Genotype & phenotype / Hematopoietic Stem Cell Transplantation / hematopoietic stem cells / Hematopoietic Stem Cells - metabolism / Hemopoiesis / Homeostasis / Humans / Immune system / Immunoglobulin G / Immunoglobulin M / Interleukin Receptor Common gamma Subunit - metabolism / Killer Cells, Natural - cytology / Killer Cells, Natural - metabolism / Kinetics / Leukocytes / Lymphocytes B / Lymphocytes T / Lymphopoiesis / Macrophages / Major histocompatibility complex / memory / Memory cells / Mice / Mice, Inbred BALB C / Mice, Inbred NOD / Myeloid cells / Natural killer cells / Phagocytes / phenotype / Protein Binding / Proteins / Receptors, Immunologic - metabolism / Receptors, Interleukin-2 - deficiency / Receptors, Interleukin-2 - metabolism / regulatory proteins / signal regulatory proteins / Signal transduction / Social interaction / Spleen / Spleen - cytology / Spleen - immunology / Stem cells / Survival Analysis / T lymphocytes / T-cell receptor / T-Lymphocytes - cytology / T-Lymphocytes - metabolism / Thymus Gland - metabolism / Transplantation, Heterologous