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Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains
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Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains
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Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains
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Journal Article
Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains
2020
Overview
Background
Coxiella burnetii
is an obligate intracellular Gram-negative bacterium that causes a zoonotic disease commonly called Q fever globally. In this study, an up-converting phosphor technology-based lateral flow (UPT-LF) assay was established for the rapid and specific detection of phase I strains of
C. burnetii
.
Results
Specific monoclonal antibodies (10B5 and 10G7) against
C. burnetii
phase I strains were prepared and selected for use in the UPT-LF assay by the double-antibody-sandwich method. The detection sensitivity of the
Coxiella
-UPT-LF was 5 × 10
4
GE/ml for a purified
C. burnetii
phase I strain and 10 ng/ml for LPS of
C. burnetii
Nine Mile phase I (NMI). Good linearity was observed for
C. burnetii
phase I and NMI LPS quantification (R
2
≥ 0.989). The UPT-LF assay also exhibited a high specificity to
C. burnetii
, without false-positive results even at 10
8
GE/ml of non-specific bacteria, and good inclusivity for detecting different phase I strains of
C. burnetii
. Moreover, the performance of the
Coxiella
-UPT-LF assay was further confirmed using experimentally and naturally infected samples.
Conclusions
Our results indicate that
Coxiella
-UPT-LF is a sensitive and reliable method for rapid screening of
C. burnetii,
suitable for on-site detection in the field.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
