Journal Article

Strain-Specific Anti-inflammatory Properties of Two Akkermansia muciniphila Strains on Chronic Colitis in Mice

Zhang, Liying, Yang, Xin, Zhai, Rui, Zhao, Liping, Xue, Xinhe, Zhang, Chenhong

2019

Overview

is potential probiotic in that its type strain ATCC BAA-835 has beneficial effects upon obesity and diabetes. However, whether can improve inflammatory bowel diseases (IBD), which is a form of chronic intestinal dysbiosis, is unknown. Hence, we used an isolated murine strain (designated 139) and type strain ATCC, to investigate their anti-inflammatory properties in cell models and in Dextran Sulfate Sodium (DSS)-induced chronic colitis of mice. , the two strains exerted similar anti-inflammatory properties as they both reduced IL-8 production by TNF-α-stimulated HT-29 cells. However, neither of the strains showed capacity to increase the differentiation of regulatory T (Treg)-cells from CD4+ T cell populations significantly. , both strains exerted anti-inflammatory effects on chronic colitis as they improved clinical parameters including spleen weight, colon inflammation index, and colon histological score. They also down-regulated the expression of the pro-inflammatory cytokines including TNF-α and IFN-γ in the colon of mice. However, the anti-inflammatory effects of strain ATCC were stronger than strain 139 in that ATCC significantly reduced spleen weight, colon inflammation index, and fecal lipocalin-2 content in mice with chronic colitis, while strain 139 was not. Dysbiosis of the gut microbiota was observed in mice with chronic colitis. Both strains facilitated the normalization of the gut microbiota. The specific capacity of strain ATCC to modulate the differentiation of Tregs as well as increase production of short chain fatty acids, demonstrated strain-specific characteristics for these two strains. This study suggests the potential beneficial effect of on IBD and the importance of the future study of the function of at the strain-level.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Akkermansia muciniphila / Animals / Anti-inflammatory agents / Anti-Inflammatory Agents - pharmacology / Antibodies / CD4 antigen / CD4-Positive T-Lymphocytes / Cell culture / Cellular and Infection Microbiology / chronic colitis / Chronic Disease / Colitis / Colitis - chemically induced / Colitis - microbiology / Colitis - pathology / Colitis - therapy / Colon / Colon - pathology / Cytokines - metabolism / Dextran / Dextran sulfate / Dextran Sulfate - adverse effects / Diabetes mellitus / Digestive system / Disease / Disease Models, Animal / DSS / Dysbacteriosis / Dysbiosis / Feces - microbiology / Flow cytometry / Gastrointestinal Microbiome / Gastrointestinal tract / Genomes / gut microbiota / HT29 Cells / Humans / Inflammatory bowel disease / Inflammatory bowel diseases / Interferon-gamma - metabolism / Interleukin-8 - metabolism / Intestinal microflora / Lipocalin / Lymphocytes T / Male / Mice / Mice, Inbred C57BL / Microbiota / Physiology / Probiotics / RNA, Ribosomal, 16S - genetics / Spleen / strain-specificity / Treg / Tumor Necrosis Factor-alpha - metabolism / Tumor necrosis factor-α / Verrucomicrobia - classification / Verrucomicrobia - genetics / Verrucomicrobia - isolation & purification / Verrucomicrobia - physiology / Whole Genome Sequencing / γ-Interferon