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Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization
by
Rodríguez, Adriana
, Vannini, Verónica
, Taranto, María P
, Sesma, Fernando
, Vera, José L
, de Valdéz, Graciela F
in
Amino Acid Sequence
/ Applied Microbiology
/ Bacteria
/ Bacterial Proteins
/ Bacterial Proteins - biosynthesis
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Base Sequence
/ biochemical pathways
/ Biochemistry
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biosynthesis
/ Biotechnology
/ chemistry
/ Cloning
/ Cloning, Molecular
/ Computer Simulation
/ enzymes
/ enzymology
/ Escherichia coli
/ Escherichia coli - genetics
/ Escherichia coli - metabolism
/ Fundamental and applied biological sciences. Psychology
/ Gene expression
/ genes
/ genetics
/ Lactobacillus reuteri
/ Lactobacillus reuteri - enzymology
/ Lactobacillus reuteri - genetics
/ Life Sciences
/ Limosilactobacillus reuteri
/ metabolism
/ Methyltransferases
/ Methyltransferases - biosynthesis
/ Methyltransferases - chemistry
/ Methyltransferases - genetics
/ Microbiology
/ Molecular Sequence Data
/ Original Research Paper
/ Recombinant Proteins
/ Recombinant Proteins - biosynthesis
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Spectrometry, Fluorescence
/ Uroporphyrinogen III Synthetase
/ Uroporphyrinogen III Synthetase - biosynthesis
/ Uroporphyrinogen III Synthetase - chemistry
/ Uroporphyrinogen III Synthetase - genetics
/ vitamin B12
2011
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Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization
by
Rodríguez, Adriana
, Vannini, Verónica
, Taranto, María P
, Sesma, Fernando
, Vera, José L
, de Valdéz, Graciela F
in
Amino Acid Sequence
/ Applied Microbiology
/ Bacteria
/ Bacterial Proteins
/ Bacterial Proteins - biosynthesis
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Base Sequence
/ biochemical pathways
/ Biochemistry
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biosynthesis
/ Biotechnology
/ chemistry
/ Cloning
/ Cloning, Molecular
/ Computer Simulation
/ enzymes
/ enzymology
/ Escherichia coli
/ Escherichia coli - genetics
/ Escherichia coli - metabolism
/ Fundamental and applied biological sciences. Psychology
/ Gene expression
/ genes
/ genetics
/ Lactobacillus reuteri
/ Lactobacillus reuteri - enzymology
/ Lactobacillus reuteri - genetics
/ Life Sciences
/ Limosilactobacillus reuteri
/ metabolism
/ Methyltransferases
/ Methyltransferases - biosynthesis
/ Methyltransferases - chemistry
/ Methyltransferases - genetics
/ Microbiology
/ Molecular Sequence Data
/ Original Research Paper
/ Recombinant Proteins
/ Recombinant Proteins - biosynthesis
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Spectrometry, Fluorescence
/ Uroporphyrinogen III Synthetase
/ Uroporphyrinogen III Synthetase - biosynthesis
/ Uroporphyrinogen III Synthetase - chemistry
/ Uroporphyrinogen III Synthetase - genetics
/ vitamin B12
2011
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Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization
by
Rodríguez, Adriana
, Vannini, Verónica
, Taranto, María P
, Sesma, Fernando
, Vera, José L
, de Valdéz, Graciela F
in
Amino Acid Sequence
/ Applied Microbiology
/ Bacteria
/ Bacterial Proteins
/ Bacterial Proteins - biosynthesis
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Base Sequence
/ biochemical pathways
/ Biochemistry
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Biosynthesis
/ Biotechnology
/ chemistry
/ Cloning
/ Cloning, Molecular
/ Computer Simulation
/ enzymes
/ enzymology
/ Escherichia coli
/ Escherichia coli - genetics
/ Escherichia coli - metabolism
/ Fundamental and applied biological sciences. Psychology
/ Gene expression
/ genes
/ genetics
/ Lactobacillus reuteri
/ Lactobacillus reuteri - enzymology
/ Lactobacillus reuteri - genetics
/ Life Sciences
/ Limosilactobacillus reuteri
/ metabolism
/ Methyltransferases
/ Methyltransferases - biosynthesis
/ Methyltransferases - chemistry
/ Methyltransferases - genetics
/ Microbiology
/ Molecular Sequence Data
/ Original Research Paper
/ Recombinant Proteins
/ Recombinant Proteins - biosynthesis
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Spectrometry, Fluorescence
/ Uroporphyrinogen III Synthetase
/ Uroporphyrinogen III Synthetase - biosynthesis
/ Uroporphyrinogen III Synthetase - chemistry
/ Uroporphyrinogen III Synthetase - genetics
/ vitamin B12
2011
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Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization
Journal Article
Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization
2011
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Overview
PURPOSE OF WORK: To clone, express and characterize uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD) from Lactobacillus reuteri. Some strains of Lb. reuteri produce cobalamin (vitamin B12). Cobalamin biosynthesis relies on the sequential action of more than 25 enzymes in a complex metabolic pathway. We have cloned, expressed and characterized the gene in Lb. reuteri that codes for the S-adenosy L-methionine uroprophyrinogen III methyltransferase/synthase (CobA/HemD), a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols.
Publisher
Springer-Verlag,Springer Netherlands,Springer,Springer Nature B.V
Subject
/ Bacteria
/ Bacterial Proteins - biosynthesis
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Biological and medical sciences
/ Biomedical and Life Sciences
/ Cloning
/ enzymes
/ Escherichia coli - metabolism
/ Fundamental and applied biological sciences. Psychology
/ genes
/ genetics
/ Lactobacillus reuteri - enzymology
/ Lactobacillus reuteri - genetics
/ Methyltransferases - biosynthesis
/ Methyltransferases - chemistry
/ Methyltransferases - genetics
/ Recombinant Proteins - biosynthesis
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Uroporphyrinogen III Synthetase
/ Uroporphyrinogen III Synthetase - biosynthesis
/ Uroporphyrinogen III Synthetase - chemistry
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