Journal Article

Malian field isolates provide insight into Plasmodium malariae intra-erythrocytic development and invasion

Cassan, Cecile, Maiga, Mohamed, Djimde, Abdoulaye A., Goita, Siaka, Dembélé, Laurent, Diakite, Ousmaila, Cazevieille, Chantal, Sogore, Fanta, Niangaly, Amadou, Marin-Menendez, Alejandro, Dabitao, Djeneba, Dao, Francois, Wague, Mamadou, Maiga, Fatoumata O., Hadara, Aboubacrin S., Talman, Arthur M.

2025

Overview

Plasmodium malariae is the third most prevalent human malaria parasite species and contributes significantly to morbidity. Nevertheless, our comprehension of this parasite’s biology remains limited, primarily due to its frequent co-infections with other species and the lack of a continuous in vitro culture system. To effectively combat and eliminate this overlooked parasite, it is imperative to acquire a better understanding of this species. In this study, we embarked on an investigation of P. malariae , including exploring its clinical disease characteristics, molecular aspects of red blood cell (RBC) invasion, and host-cell preferences. We conducted our research using parasites collected from infected individuals in Mali. Our findings revealed anaemia in most of P. malariae infected participants presented, in both symptomatic and asymptomatic cases. Regarding RBC invasion, quantified by an adapted flow cytometry based method, our study indicated that none of the seven antibodies tested, against receptors known for their role in P. falciparum invasion, had any impact on the ability of P. malariae to penetrate the host cells. However, when RBCs were pre-treated with various enzymes (neuraminidase, trypsin, and chymotrypsin), we observed a significant reduction in P. malariae invasion, albeit not a complete blockade. Furthermore, in a subset of P. malariae samples, we observed the parasite’s capability to invade reticulocytes. These results suggest that P. malariae employs alternative pathways to enter RBCs of different maturities, which may differ from those used by P. falciparum .

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adolescent / Adult / Anemia - parasitology / Biology and Life Sciences / Causes of / Child / Development and progression / Ecology and Environmental Sciences / Ecology, environment / Erythrocytes / Erythrocytes - parasitology / Female / Health / Health aspects / Human health and pathology / Humans / Infection / Infectious diseases / Life Sciences / Malaria / Malaria - epidemiology / Malaria - parasitology / Male / Mali - epidemiology / Medicine and Health Sciences / Middle Aged / Physiological aspects / Plasmodium / Plasmodium falciparum / Plasmodium malariae - growth & development / Plasmodium malariae - isolation & purification / Plasmodium malariae - physiology / Research and Analysis Methods / Symbiosis / Young Adult