Asset Details
Do you wish to reserve the book?
Dynamic Profiling of Antitumor Activity of CAR T Cells Using Micropatterned Tumor Arrays
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Dynamic Profiling of Antitumor Activity of CAR T Cells Using Micropatterned Tumor Arrays
Do you wish to request the book?
Dynamic Profiling of Antitumor Activity of CAR T Cells Using Micropatterned Tumor Arrays
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Dynamic Profiling of Antitumor Activity of CAR T Cells Using Micropatterned Tumor Arrays
2019
Overview
Cancer immunotherapy based on the engineering of chimeric antigen receptors (CAR) on T cells has emerged as one of the most promising new therapies for patients with B‐cell malignancies. Preclinical assessments of essential CAR T cell functions such as trafficking and cytotoxicity are critical for accelerating the development of highly effective therapeutic candidates. However, current tools for evaluating CAR‐T functions lack sufficient precision. Here, a micropatterned tumor array (MiTA) is described that enables detailed and dynamic characterization of CAR T cell trafficking toward tumor‐cell islands and subsequent killing of tumor cells. It is shown that CAR T cells often merge into large clusters that envelop and kill the tumor cells with high efficiency. Significant differences are also measured between CAR T cells from different donors and between various CAR T cell constructs. Overall, the assay allows for multifaceted, dynamic, high‐content evaluation of CAR T trafficking, clustering, and killing and could eventually become a useful tool for immune‐oncology research and preclinical assessments of cell‐based immunotherapies. A micropatterned tumor array enables multifaceted and dynamic characterization of antitumor activity of CAR T cells. The assay demonstrates that CAR T cells migrate and merge into clusters that envelop the tumor islands and eliminate the tumor cells. It reveals significant differences in antitumor function between CAR T cells from different donors and between different CAR T cell constructs.
Publisher
John Wiley & Sons, Inc,John Wiley and Sons Inc,Wiley
Subject
