Journal Article

Identification of a Mannitol Transporter, AgMaT1, in Celery Phloem

Lemoine, Rémi, Noiraud, Nathalie, Maurousset, Laurence

2001

Overview

A celery petiole phloem cDNA library was constructed and used to identify a cDNA that gives Saccharomyces cerevisiae cells the ability to grow on mannitol and transport radiolabeled mannitol in a manner consistent with a proton symport mechanism. This cDNA was named AgMaT1 (Apium graveolens mannitol transporter 1). The expression profile in source leaves and phloem was in agreement with a role for mannitol in phloem loading in celery. The identification in eukaryotes of a mannitol transporter is important because mannitol is not only a primary photosynthetic product in species such as celery but is also considered a compatible solute and antioxidant implicated in resistance to biotic and abiotic stress.

• Publisher: American Society of Plant Physiologists,American Society of Plant Biologists
• Subject: active transport / AgMat1 gene / Amino Acid Sequence / amino acid sequences / Apiaceae / Apiaceae - metabolism / Apium graveolens / Base Sequence / Carrier Proteins / Carrier Proteins - genetics / Carrier Proteins - metabolism / Celery / Cloning, Molecular / Complementary DNA / Dehydrogenases / Escherichia coli / Gene Expression Regulation / Gene Library / gene transfer / genetic transformation / genetics / mannitol / Mannitol - metabolism / mannitol transporter / Membrane Proteins / Membrane Proteins - genetics / Membrane Proteins - metabolism / Membrane Transport Proteins / messenger RNA / metabolism / Molecular Sequence Data / Nucleic Acid Amplification Techniques / nucleotide sequences / Organisms, Genetically Modified / Petioles / Phloem / Phylogeny / Plant cells / Plant Proteins / Plants / plasma membrane / RNA / Saccharomyces / Saccharomyces cerevisiae / Saccharomyces cerevisiae - genetics / Sequence Homology / Substrate Specificity / Vegetables / Yeasts