Journal Article

Perirenal Adipose Tissue Displays an Age-Dependent Inflammatory Signature Associated With Early Graft Dysfunction of Marginal Kidney Transplants

Simoncini, Stephanie, Meunier, Maité, Arnaud, Laurent, Legris, Tristan, Lyonnet, Luc, Lechevallier, Eric, Gondran Tellier, Bastien, François, Pauline, Boissier, Romain, Sabatier, Florence, Dignat George, Françoise, Magalon, Jeremy, Paul, Pascale, Karsenty, Gilles, Giraudo, Laurent, Burtey, Stéphane

2020

Overview

Better understanding of the contribution of donor aging and comorbidity factors of expanded criteria donors (ECD) to the clinical outcome of a transplant is a challenge in kidney transplantation. We investigated whether the features of donor-derived stromal vascular fraction of perirenal adipose tissue (PRAT-SVF) could be indicative of the deleterious impact of the ECD microenvironment on a renal transplant. A comparative analysis of cellular components, transcriptomic and vasculogenic profiles was performed in PRAT-SVF obtained from 22 optimal donors and 31 ECD deceased donors. We then investigated whether these parameters could be associated with donor aging and early allograft dysfunction. When compared with the PRAT-SVF of non-ECD donors, ECD PRAT-SVF displayed a lower proportion of stromal cells, a higher proportion of inflammatory NK cells. The global RNA sequencing approach indicated a differential molecular signature in the PRAT-SVF of ECD donors characterized by the over-expression of CXCL1 and IL1-β inflammatory transcripts. The vasculogenic activity of PRAT-SVF was highly variable but was not significantly affected in marginal donors. Periorgan recruitment of monocytes/macrophages and NK cells in PRAT-SVF was associated with donor aging. The presence of NK cell infiltrates was associated with lower PRAT-SVF angiogenic activity and with early allograft dysfunction evaluated on day 7 and at 1 month post-transplant. Our results indicate that human NK cell subsets are differentially recruited in the periorgan environment of aging kidney transplants. We provide novel evidence that PRAT-SVF represents a non-invasive and timely source of donor material with potential value to assess inflammatory features that impact organ quality and function.

• Publisher: Frontiers Media SA,Frontiers,Frontiers Media S.A
• Subject: Adipose tissue / Adipose Tissue - physiology / Adult / Aged / Aging / Allografts / Angiogenesis / Antibodies / Antigens / Blood & organ donations / Body fat / Cell Movement / Chemokine CXCL1 - genetics / Chemokine CXCL1 - metabolism / Comorbidity / Comparative analysis / Creatinine / Donors / endothelial inflammation / Female / Flow cytometry / Gene expression / Humans / Hypertension / Immunology / Inflammation / Inflammation - immunology / Interleukin-1beta - genetics / Interleukin-1beta - metabolism / Kidney - immunology / kidney allograft dysfunction / Kidney Transplantation / Kidney transplants / Killer Cells, Natural - immunology / Life Sciences / Macrophages / Macrophages - immunology / Male / marginal kidney donors / Microenvironments / Middle Aged / Monocytes / Natural killer cells / Neovascularization, Pathologic / Overexpression / perirenal adipose tissue / Primary Graft Dysfunction - immunology / Prospective Studies / Risk factors / Stromal cells / Tissue Donors / Transcriptome / Transcriptomics / Transplants