Journal Article

Dynamics of photosystem II: a proteomic approach to thylakoid protein complexes

Suorsa, M, Allahverdiyeva, Y, Rokka, A, Saleem, A, Rintamäki, E, Battchikova, N, Paakkarinen, V, Aro, E-M

2005

Overview

Oxygenic photosynthesis produces various radicals and active oxygen species with harmful effects on photosystem II (PSII). Such photodamage occurs at all light intensities. Damaged PSII centres, however, do not usually accumulate in the thylakoid membrane due to a rapid and efficient repair mechanism. The excellent design of PSII gives protection to most of the protein components and the damage is most often targeted only to the reaction centre D1 protein. Repair of PSII via turnover of the damaged protein subunits is a complex process involving (i) highly regulated reversible phosphorylation of several PSII core subunits, (ii) monomerization and migration of the PSII core from the grana to the stroma lamellae, (iii) partial disassembly of the PSII core monomer, (iv) highly specific proteolysis of the damaged proteins, and finally (v) a multi-step replacement of the damaged proteins with de novo synthesized copies followed by (vi) the reassembly, dimerization, and photoactivation of the PSII complexes. These processes will shortly be reviewed paying particular attention to the damage, turnover, and assembly of the PSII complex in grana and stroma thylakoids during the photoinhibition–repair cycle of PSII. Moreover, a two-dimensional Blue-native gel map of thylakoid membrane protein complexes, and their modification in the grana and stroma lamellae during a high-light treatment, is presented.

• Publisher: Oxford University Press,Oxford Publishing Limited (England)
• Subject: Arabidopsis thaliana / Arabidopsis thylakoid membrane proteome / assembly of photosystem II / Biochemistry / Biological and medical sciences / Chloroplasts / D1 protein / free radicals / Fundamental and applied biological sciences. Psychology / Grana / Light / light intensity / light stress / Light-Harvesting Protein Complexes / Light-Harvesting Protein Complexes - metabolism / literature reviews / Mechanisms of Light-Induced Damage / Metabolism / Monomers / Oxidation-Reduction / oxidative stress / Oxygen / Oxygen - metabolism / Phosphorylation / Photoactivation / photoinhibition / photooxidation / Photosynthesis / Photosynthesis, respiration. Anabolism, catabolism / Photosystem II / photosystem II photoinhibition / Photosystem II Protein Complex / Photosystem II Protein Complex - metabolism / Photosystem II Protein Complex - physiology / Photosystem II Protein Complex - radiation effects / physiology / Plant physiology and development / Plants / protein degradation / protein metabolism / protein phosphorylation / Proteins / proteomics / radiation effects / reactive oxygen species / repair of photosystem II / Thylakoids / Thylakoids - metabolism / Thylakoids - radiation effects