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Introducing a salt bridge into the lipase of Stenotrophomonas maltophilia results in a very large increase in thermal stability
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Introducing a salt bridge into the lipase of Stenotrophomonas maltophilia results in a very large increase in thermal stability
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Journal Article
Introducing a salt bridge into the lipase of Stenotrophomonas maltophilia results in a very large increase in thermal stability
2015
Overview
High thermostability of enzymes is a prerequisite for their biotechnological applications. An organic solvent-tolerant and cold-active lipase, from the Stenotrophomonas maltophilia, was unstable above 40 °C in previous studies. To increase the enzyme stability, possible hydrogen-bond networks were simulated by the introduction of a salt bridge in a highly flexible region of the protein. Compared with the wild-type lipase, a mutant lipase (G165D and F73R) showed a >900-fold improvement in half-life at 50 °C, with the optimal activity-temperature increasing from 35 to 90 °C. Therefore, the hydrogen-bond strategy is a powerful approach for improving enzyme stability through the introduction of a salt bridge.
Publisher
Springer-Verlag,Springer Netherlands,Springer Nature B.V
Subject
/ Bacteria
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biomedical and Life Sciences
/ Enzymes
/ Lipase
/ Molecular Dynamics Simulation
/ mutants
/ Networks
/ Salts
/ Stenotrophomonas maltophilia
/ Stenotrophomonas maltophilia - enzymology
/ Stenotrophomonas maltophilia - genetics
/ Strategy
