Journal Article

A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019

Wu, Gui-cheng, Long, Quan-xin, Chen, Yao-kai, Tang, Ni, He, Chang-long, Lin, Yong, Tian, Wen-guang, Wang, De-qiang, Li, Zhi-jie, Fan, Kai, Zhang, Xiao-li, Wei, Jie, Gan, Chun-yang, Du, Hong-xin, Deng, Hai-jun, Liao, Pu, Hu, Yuan, Zhou, Fa-Chun, Wang, Dao-Xin, Huang, Ai-long, Hu, Jie- li, Liu, Ping, Xiang, Jiang-lin, Hu, Peng, Chen, A-mei, Chen, Yan-meng, Wang, Kun, Wang, Jing, Huang, Lu-yi, Ren, Ji-hua, Gao, Qing-zhu, Cai, Xue-fei, Liu, Bei-zhong, Chen, Juan

2020

Overview

Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. Methods In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. Results To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. Conclusions Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19. A peptide-based magnetic chemiluminescence enzyme immunoassay for the detection of SARS-CoV-2 antibodies was developed; 71.4% (197 of 276) and 57.2% (158 of 276) of the COVID-19 inpatients were positive for IgG and IgM against SARS-CoV-2.

• Publisher: Oxford University Press
• Subject: Adult / Antibodies, Viral - blood / Betacoronavirus - immunology / Chemiluminescence / Clinical Laboratory Techniques - methods / Coronaviridae / Coronavirus Infections - diagnosis / Coronavirus Infections - immunology / Coronaviruses / COVID-19 / COVID-19 Testing / COVID-19 Vaccines / COVID-2019 / Enzyme immunoassay / Female / Humans / Immunoassay / Immunoenzyme Techniques - methods / Immunoglobulin G / Immunoglobulin G - blood / Immunoglobulin M / Immunoglobulin M - blood / Luminescent Measurements / Major / Male / Medical diagnosis / Middle Aged / Pandemics / Peptides / Peptides - immunology / Pneumonia, Viral - diagnosis / Pneumonia, Viral - immunology / Polymerase chain reaction / Real-Time Polymerase Chain Reaction / Reverse Transcriptase Polymerase Chain Reaction / SARS-CoV-2 / Sensitivity and Specificity / Serologic Tests - methods / Severe acute respiratory syndrome coronavirus 2 / Viral Proteins - immunology